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Tracking Particle-Encapsulated DNA Across the Anion-Exchange Chromatography Fractions of Recombinant Adeno-Associated Virus Using Droplet Digital PCR and High-Throughput Sequencing. [PDF]
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Ion-exchange chromatography for the characterization of biopharmaceuticals
Journal of Pharmaceutical and Biomedical Analysis, 2015Ion-exchange chromatography (IEX) is a historical technique widely used for the detailed characterization of therapeutic proteins and can be considered as a reference and powerful technique for the qualitative and quantitative evaluation of charge heterogeneity.
Fekete, Szabolcs +3 more
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Current Protocols in Protein Science, 1998
AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
Richard J. FitzGerald, David Sheehan
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AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
Richard J. FitzGerald, David Sheehan
openaire +7 more sources
Ion-exchange chromatography [PDF]
Analytical Chemistry, 1964 Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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Biofuels, Bioproducts and Biorefining, 2019
The consumption and production of biodiesel has grown dramatically in recent decades, motivated by a strong interest in renewable energy. This has generated a surplus of its main by‐product, glycerol.
M. Arcanjo +7 more
semanticscholar +1 more source
The consumption and production of biodiesel has grown dramatically in recent decades, motivated by a strong interest in renewable energy. This has generated a surplus of its main by‐product, glycerol.
M. Arcanjo +7 more
semanticscholar +1 more source
Journal of Separation Science, 2016
The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare.
Simon Kluters +3 more
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The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare.
Simon Kluters +3 more
semanticscholar +1 more source
Analytical Chemistry, 1962
Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, Francis X. McGarvey
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Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, Francis X. McGarvey
openaire +4 more sources