Results 291 to 300 of about 536,774 (369)

Characterization of intact protein conjugates and biopharmaceuticals using ion-exchange chromatography with online detection by native electrospray ionization mass spectrometry and top-down tandem mass spectrometry.

Analytical Chemistry, 2015
Characterization of biopharmaceutical products is a challenging task, which needs to be carried out at several different levels (including both primary structure and conformation).
Khaja Muneeruddin, Mark Nazzaro, I. Kaltashov   +2 more
semanticscholar   +1 more source

On-Line Ion Exchange Liquid Chromatography as a Process Analytical Technology for Monoclonal Antibody Characterization in Continuous Bioprocessing.

Analytical Chemistry, 2017
Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes.
Bhumit A. Patel, N. D. Pinto, A. Gospodarek, Bruce R Kilgore, K. Goswami, William N. Napoli, Jayesh Desai, J. Heo, Dominick Panzera, D. Pollard, Daisy Richardson, Mark Brower, D. Richardson   +12 more
semanticscholar   +1 more source

Modeling of salt and pH gradient elution in ion-exchange chromatography.

Journal of Separation Science, 2014
The separation of proteins by internally and externally generated pH gradients in chromatofocusing on ion-exchange columns is a well-established analytical method with a large number of applications.
Michael Schmidt, M. Hafner, Christian Frech   +2 more
semanticscholar   +1 more source

[3] Ion-exchange chromatography

1996
Publisher Summary Over the years, electrophoretic techniques were the leading bioanalytical tools for biopolymers. Slab gel electrophoresis has yielded spectacular results, in resolving closely related nucleic acids of high molecular mass. Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) have served well ...
Gargi Choudhary, Csaba Horváth
openaire   +3 more sources

Expanded and fixed bed ion exchange chromatography for the recovery of C‐phycocyanin in a single step by using lysed cells

, 2015
C-phycocyanin, a natural blue dye, stands out today because it has antioxidant, anti-inflammatory, and anti-tumor properties, and it can be extracted from cyanobacteria, such as the genus Spirulina. A process that has received attention is the adsorption
C. C. Moraes, L. Sala, J. C. Ores, A. R. Braga, J. Costa, S. Kalil   +5 more
semanticscholar   +1 more source

Ion-exchange chromatography

Analytica Chimica Acta, 1967
Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
openaire   +3 more sources

Chapter 22 Ion-Exchange Chromatography

2009
Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois Jungbauer, Rainer Hahn
openaire   +3 more sources

THE PURIFICATION OF TRYPSINOGEN BY ION-EXCHANGE CHROMATOGRAPHY

Canadian Journal of Biochemistry and Physiology, 1962
The purification of trypsinogen by several methods of ion-exchange chromatography has been investigated. The use of phosphate–urea buffers on Bio-Rex 70 was not a suitable preparative procedure because of considerable inactivation of the zymogen. Chromatography on carboxymethyl cellulose at pH 3.2 produced no significant increase in the potential ...
G. Y. Mabbott, Lawrence B. Smillie, G. R. Neufeld   +2 more
openaire   +3 more sources

High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.

Journal of Separation Science, 2013
Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers
Kecheng Li, Song Liu, R. Xing, Huahua Yu, Yukun Qin, Rongfeng Li, Pengcheng Li   +6 more
semanticscholar   +1 more source

Robust mechanistic modeling of protein ion-exchange chromatography.

Journal of Chromatography A, 2021
Vijesh Kumar, Samuel Leweke, W. Heymann, E. von Lieres, F. Schlegel, Karin Westerberg, A. Lenhoff   +6 more
semanticscholar   +1 more source