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AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
Richard J. FitzGerald, David Sheehan
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Ion-exchange chromatography [PDF]
Analytical Chemistry, 1964 Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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