Total plasma homocysteine measurement: Evaluation of the Abbott immunoassay, comparison with the JEOL ion exchange chromatography and investigation of its clinical utility [PDF]
Practical Laboratory Medicine, 2022 Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen +6 more
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Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]
BiomedicinesBackground: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
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Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration.
Jelena Ruscic +4 more
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Antibody Aggregate Removal by Multimodal Chromatography [PDF]
MoleculesThe growing demand for therapeutic monoclonal antibodies (mAbs) has heightened the need for efficient and scalable purification strategies. A major challenge in downstream processing is the removal of antibody aggregates, which can compromise drug safety,
Veronika Rupčíková +3 more
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PURIFICATION AND PARTIAL CHARACTERIZATION OF SERUM IMMUNOGLOBULIN FROM PERSIAN STURGEON (ACIPENSER PERSICUS) [PDF]
Majallah-i ̒Ilmī-i Shīlāt-i Īrān, 2003 In this study, immunoglobulins from serum of Persian sturgeon (Acipenser persicus) were purified and partially characterized. Immunoglobulins were purified from the pooled sera by a combination of salt precipitation, Ion-exchange chromatography and gel ...
M. Kalbasi1; A. Mostafaei2; J. Majidi3
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Effect of ion-exchange chromatography on removal of fibronectin in von Willebrand factor
Zhongguo shuxue zazhi, 2023 Objective To study the removal effect of fibronectin(Fn) from von willebrand factor(vWF) by ion-exchange chromatography through processing human coagulation factor Ⅷ chromatographic washing products, in order to select a method that can effectively ...
Zheng ZHOU +4 more
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The Role of Vibrio cholerae Haemagglutinin Protease (HAP) in Extra-Intestinal Infection [PDF]
Journal of Clinical and Diagnostic Research, 2016 Introduction: Based on the diversity of surface O antigen Vibrio cholerae can be classified into 206 serogroups. Vibrio cholerae is the causative agent of cholera and extra intestinal infections like, septicemia, wound infection and haemorrhagic ...
Amit Ghosh, Hemanta Koley, Amit Pal
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The role of lactic acid adsorption by ion exchange chromatography. [PDF]
PLoS ONE, 2010 BACKGROUND: The polyacrylic resin Amberlite IRA-67 is a promising adsorbent for lactic acid extraction from aqueous solution, but little systematic research has been devoted to the separation efficiency of lactic acid under different operating conditions.
Qiang Gao +7 more
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Czech Journal of Food Sciences, 2011 An ion-exchange chromatography method with visible light detection was developed for the simultaneous determination of chloride, bromide, and iodide in foodstuffs.
Lingyun Yu +4 more
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Isolation and characterisation of metallothionein from the clam Ruditapes decussatus [PDF]
, 2003 Metallothioneins (MT) were obtained after purification from metal-exposed clams (Ruditapes decussatus) using gel-permeation and ion-exchange chromatography.
Bebianno +42 more
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