Results 91 to 100 of about 3,527,182 (276)

Improvement in protein separation by pH excursion modulated ion-exchange chromatography

Journal of Chromatography Open
The transient change in pH during the elution step in ion exchange chromatography is commonly referred to as pH excursion. Acidic pH excursion is usually observed during salt-based elution in cation exchange chromatography.
Raja Ghosh
doaj   +1 more source

A Cu2S/NiOOH Heterojunction Enables Highly Selective Ammonia‐to‐Nitrite Electrooxidation at Industrially Relevant Current Densities

Advanced Materials, EarlyView.
Cu2S/NiOOH heterojunction enables efficient ammonia electrooxidation coupled with hydrogen evolution. Interfacial electronic modulation of Ni sites promotes selective NH3‐to‐NO2− electrooxidation at high current density. In a membrane electrode assembly, Cu2S/NiOOH maintains over 90% Faradaic efficiency of nitrite at 800 mA cm−2, highlighting its ...
Ke Wang, Hongzhe Anna Xu, Xin Mao, Xuefei Wang, Min Zheng, Chun‐Chuan Kao, Limei Yang, Jiashu Wang, Boming Sun, Shokoofeh Varshooy, Aijun Du, Qi‐Long Zhu, Jian Liu, Zhenguo Huang   +13 more
wiley   +1 more source

Adaptive Supramolecular Interactions Govern Redox‐Active Molecules in Aqueous Redox Flow Batteries

Advanced Materials, EarlyView.
Adaptive supramolecular interactions overcome the solubility‐stability trade‐off in aqueous redox flow batteries. Hydrophobic anions disrupt intrinsic π–π stacking to enable high solubility, while redox‐state‐dependent spin pairing and cation coordination dynamically stabilize reactive intermediates during operation.
Vikram Singh, Seongyeon Kwon, Giu A. Silva Testa, Ariyeong Yun, Yohan Kim, Yunseop Choi, Dongwook Kim, Seongmo Ahn, Minyoung Shim, Jongcheol Seo, Evan Wenbo Zhao, Mu−Hyun Baik, Hye Ryung Byon   +12 more
wiley   +1 more source

Enhancing Selectivity of Protein Biopharmaceuticals in Ion Exchange Chromatography through Addition of Organic Modifiers. [PDF]

Int J Mol Sci, 2023
Duivelshof BL, Bouvarel T, Pirner S, Larraillet V, Knaupp A, Koll H, D'Atri V, Guillarme D.   +7 more
europepmc   +1 more source

Experimental and numerical modelling comparative study of ion-exchange chromatography [PDF]

, 2004
Chromatography is increasingly being used for large-scale preparative separation of precious biological components such as recombinant proteins and DNA. Several kinds of mathematical models are used to describe the liquid chromatography process.
Jozwik, Magdalena
core   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

Advanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green, Michael W. Jones, Merissa Saleem, Haobo Ge, Melita Tvardauskaite, Julia Dudzic, Fernando Cortezon‐Tamarit, Rory L. Arrowsmith, Charareh Pourzand, Gabriele Kociock‐Kohn, Nicholas H. Rees, Jonathan R. Dilworth, Stephen Faulkner, Stanley W. Botchway, David Gurevich, Sofia I. Pascu   +15 more
wiley   +1 more source

A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides

Oligonucleotides are commonly purified using either ion exchange chromatography (IEX) or ion-pair reversedphase liquid chromatography (IP-RPLC). This study compares the purification of a crude 20-mer oligonucleotide (ON) using both methods under ...
Thunberg, Linda, Fornstedt, Torgny, Unoson, Cecilia,, Samuelsson, Jörgen, Stavenhagen, Kathrin,, Borland, Kayla,, Unoson, Cecilia, Jora, Manasses, Samuelsson, Jörgen,, Stavenhagen, Kathrin, Stalberg, Olof,, Leek, Tomas, Jora, Manasses,, Borland, Kayla, Leek, Tomas,, Lesko, Marek, Stalberg, Olof, Enmark, Martin,, Lesko, Marek,, Thunberg, Linda,, Fornstedt, Torgny,, Enmark, Martin   +21 more
core   +1 more source

Innate Immunocompetent hiPSC‐Derived Neurospheroids Capture Early CNS Responses to rAAV

Advanced Science, EarlyView.
Knowledge of human CNS immune responses to AAV‐based gene therapies remains limited due to the lack of immune‐competent human models. Here, a hiPSC‐derived 3D neuroimmune platform integrating neurospheroids and microglia is established using stirred‐tank bioreactors.
Catarina M. Gomes, Gabriela Silva, Mafalda Aleixo, Marta Gomes, Daniel Simão, Stephan J. Holtkamp, Diana D. Lobo, Pradeep Harish, Rosalind Jenkins, Lekh N. Dahal, Rui J. Nobre, Luís de Pereira de Almeida, Mark Trautwein, Paula M. Alves, Catarina Brito   +14 more
wiley   +1 more source

Insights Into CO2 Loss, pH Effects, and Tafel Kinetics in Ni Single Atom‐Driven Bicarbonate Electroreduction

Advanced Science, EarlyView.
Selective CO formation in bicarbonate electrolysis is achieved by retaining reactive CO2 and tuning pH, while the competing hydrogen evolution reaction occurs at the interface. The rate‐determining step couples the chemical equilibrium of bicarbonate/CO2 with electrochemical charge transfer, offering insight into optimizing reaction conditions for ...
Lin Li, Yi‐Jie Kong, Ting Zhang, Xu Han, Kristine Aalestrup, Steen Uttrup Pedersen, Xin‐Ming Hu, Kim Daasbjerg   +7 more
wiley   +1 more source

Purification of bovine pancreatic glucagon as a by-product of insulin production

Brazilian Journal of Medical and Biological Research, 1997
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation.
A.S.R. Andrade, L. Vilela, H. Tunes
doaj   +1 more source