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Current Protocols in Protein Science, 1998
AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
A, Williams, V, Frasca
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AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
A, Williams, V, Frasca
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Methods in enzymology, 2010
Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois, Jungbauer, Rainer, Hahn
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Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois, Jungbauer, Rainer, Hahn
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Analytical Chemistry, 1962
Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, F. X. McGarvey
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Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, F. X. McGarvey
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Analytical Chemistry, 1964
Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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Analytica Chimica Acta, 1967
Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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1979
Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Antibody Purification: Ion-Exchange Chromatography
2009Ion exchange chromatography techniques are the focus of this chapter and they showcase the power of this method for the purification of proteins and monoclonal antibodies. The technique is powerful and can separate biomolecules that have minor differences in their net charge, e.g., two protein molecules differing by a single charged amino acid.
Ana Cristina, Grodzki, Elsa, Berenstein
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Ion Exchange in Paper Chromatography
Nature, 1953IT has been shown by Schute that several mixtures of alkaloids can be separated by chromatography on paper using aqueous ammonia1. Because the chromatograms have the characteristics of a partition chromatogram, it is probable that the above separation is due to partition between an aqueous imbibition phase and an aqueous mobile phase.
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Ion-Exchange Chromatography in Alcohol
Nature, 1955IN the technique of ion-exchange chromatography, the order in which compounds are eluted from a column depends basically upon differences in their ionization constants; but other factors modify and in many instances reverse the expected order1. This is illustrated in Fig.
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