Global and Local Conformation of Human IgG Antibody Variants Rationalizes Loss of Thermodynamic Stability. [PDF]
, 2015 Immunoglobulin G (IgG) monoclonal antibodies (mAbs) are a major class of medicines, with high specificity and affinity towards targets spanning many disease areas. The antibody Fc (fragment crystallizable) region is a vital component of existing antibody
Edgeworth, Matthew J +5 more
core +1 more source
Prediction of fibril formation by early-stage amyloid peptide aggregation
Journal of Pharmaceutical Analysis, 2020 Amyloid fibrils are found in systemic amyloidosis diseases such as Alzheimer’s disease, Parkinson’s disease, and type II diabetes. Currently, these diseases are diagnosed by observation of fibrils or plaques, which is an ineffective method for early ...
Jiaojiao Hu +3 more
doaj +1 more source
Integrating ion mobility mass spectrometry with molecular modelling to determine the architecture of multiprotein complexes. [PDF]
PLoS ONE, 2010 Current challenges in the field of structural genomics point to the need for new tools and technologies for obtaining structures of macromolecular protein complexes.
Argyris Politis +5 more
doaj +1 more source
Identification of Pesticides Using Experimental and Computational Approaches based on Ion Mobility Mass Spectrometry Measurements [PDF]
, 2022 Mass spectrometry is known to be a powerful analytical technique due its high sensitivity, specificity, speed, and versatility. It has the capability of detecting and identifying numerous analytes in sample matrices.
Benedicta, Donkor
core
Characterization of the Metal-Binding Sites of Metallothioneins and Structural Changes Between Metal-Free and Metal-Bound Forms by Ion Mobility-Mass Spectrometry (IM-MS) [PDF]
Biophysical Journal, 2011 Metal ions play important roles in many chemical and biochemical processes, such as oxidation, oxygen transport, and electron transfer. The cellular trafficking of metal ions is controlled by a group of low molecular weight (6-10 kDa), cysteine-rich proteins (∼30%) referred to as metallothioneins (MTs).
Chen, Shu-Hua, Russell, David H.
openaire +1 more source
IR action spectroscopy of glycosaminoglycan oligosaccharides [PDF]
, 2020 Glycosaminoglycans (GAGs) are a physio- and pharmacologically highly relevant class of complex saccharides, possessing a linear sequence and strongly acidic character.
Freyse, Joanna +9 more
core +2 more sources
Insights into ultra-low affinity lipase-antibody noncovalent complex binding mechanisms
mAbs, 2022 Detection of host cell protein (HCP) impurities is critical to ensuring that recombinant drug products, including monoclonal antibodies (mAbs), are safe.
Elizabeth Sara Hecht +11 more
doaj +1 more source
Plate-height model of ion mobility-mass spectrometry [PDF]
, 2020 In the past decade, ion mobility spectrometry (IMS) in combination with mass spectrometry (IM-MS) became a widely employed technique for the separation and structural characterization of ionic species in the gas phase.
Causon, T. +5 more
core +2 more sources
Investigation of Dendriplexes by Ion Mobility-Mass Spectrometry
Molecules, 2014 Highly branched polyamidoamine (PAMAM) dendrimers presenting biological activities have been envisaged as non-viral gene delivery vectors. They are known to associate with nucleic acid (DNA) in non-covalent complexes via electrostatic interactions ...
Emma-Dune Leriche +6 more
doaj +1 more source
Software Tools and Approaches for Compound Identification of LC-MS/MS Data in Metabolomics. [PDF]
, 2018 The annotation of small molecules remains a major challenge in untargeted mass spectrometry-based metabolomics. We here critically discuss structured elucidation approaches and software that are designed to help during the annotation of unknown compounds.
Blaženović, Ivana +3 more
core +3 more sources