Results 51 to 60 of about 30,983 (257)

Electron transfer with no dissociation ion mobility–mass spectrometry (ETnoD IM-MS). The effect of charge reduction on protein conformation

International Journal of Mass Spectrometry, 2017
A novel mass spectrometry approach is reported which investigate how ion-molecule charge reduction reactions between radical anions and protein cations modulate protein conformation. An electron transfer reagent (1,3-dicyanobenzene) transfers electrons to positively charged proteins and there are no observable products of dissociation.
Jacquelyn R. Jhingree, Rebecca Beveridge, Eleanor R. Dickinson, Jonathan P. Williams, Jeffery M. Brown, Bruno Bellina, Perdita E. Barran   +6 more
openaire   +4 more sources

Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts

, 2020
A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS).
Barsch, A., Brunner, A., Mann, M., Meier, F., Meitei, N., Meyer, S., Schweiger-Hufnagel, U., Sulek, K., Vasilopoulou, C.   +8 more
core   +1 more source

A comparison of collision cross section values obtained via travelling wave ion mobility-mass spectrometry and ultra high performance liquid chromatography-ion mobility-mass spectrometry : application to the characterisation of metabolites in rat urine [PDF]

, 2019
A comprehensive Collision Cross Section (CCS) library was obtained via Travelling Wave Ion Guide mobility measurements through direct infusion (DI). The library consists of CCS and Mass Spectral (MS) data in negative and positive ElectroSpray Ionisation (
Bouwmeester, Robbin, Coen, Muireann, Gethings, Lee A, Lai, Steven, Langridge, James I, Letertre, Marine PM, Martens, Lennart, McCullagh, Michael, Munjoma, Nyasha C, Nicholson, Jeremy K, Nye, Leanne C, Plumb, Robert S, Swann, Jonathan R, Vissers, Johannes PC, Williams, Jonathan P, Wilson, Ian D   +15 more
core   +2 more sources

Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase

Scientific Reports, 2018
Ion mobility mass spectrometry (IM-MS) is a technique capable of investigating structural changes of biomolecules based on their collision cross section (CCS).
Suyong Re, Shigehisa Watabe, Wataru Nishima, Eiro Muneyuki, Yoshiki Yamaguchi, Alexander D. MacKerell, Yuji Sugita   +6 more
doaj   +1 more source

Integrated Metabolome and Lipidome Strategy to Reveal the Action Pattern of Paclobutrazol, a Plant Growth Retardant, in Varying the Chemical Constituents of Platycodon Root

Molecules, 2022
Platycodon root, a medicinal food homology species which has been used in Asian countries for hundreds of years, is now widely cultivated in China. Treatment with paclobutrazol, a typical plant growth retardant, has raised uncertainties regarding the ...
Lan Lan, Weizhen Huang, Heng Zhou, Jiajia Yuan, Shui Miao, Xiuhong Mao, Qing Hu, Shen Ji   +7 more
doaj   +1 more source

The role of the mobile proton in fucose migration [PDF]

, 2019
Fucose migration reactions represent a substantial challenge in the analysis of fucosylated glycan structures by mass spectrometry. In addition to the well-established observation of transposed fucose residues in glycan-dissociation product ions, recent ...
Helden, Gert von, Lettow, Maike, Manz, Christian, Marianski, Mateusz, Meijer, Gerard, Mucha, Eike, Pagel, Kevin, Thomas, Daniel A.   +7 more
core   +1 more source

A Rapid and Sensitive Method for the Simultaneous Determination of Multipolar Compounds in Plant Tea by Supercritical Fluid Chromatography Coupled to Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry

Foods, 2022
In this study, matrix solid phase dispersion (MSPD) microextraction combined with supercritical fluid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (SFC/IM-QTOF-MS) was used to analyze the multipolar compounds in plant tea.
Zi-Xuan Yue, Jun Cao
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling [PDF]

, 2015
© 2015 Mitchell et al. Abstract Background: Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents ...
Clench, Malcolm, Donaldson, M., Francese, S., Long, H., Mitchell, C.A.   +4 more
core   +2 more sources