Results 51 to 60 of about 2,450,645 (310)

Coulombic Effects in Ion Mobility Spectrometry [PDF]

Analytical Chemistry, 2009
Ion mobility spectrometry (IMS) has been increasingly employed in a number of applications. When coupled to mass spectrometry (MS), IMS becomes a powerful analytical tool for separating complex samples and investigating molecular structure. Therefore, improvements in IMS-MS instrumentation, e.g., IMS resolving power and sensitivity, are highly ...
Tolmachev, Aleksey V, Clowers, Brian H, Belov, Mikhail E, Smith, Richard D   +3 more
openaire   +3 more sources

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Application of Ion Mobility Mass Spectrometry in Food Analysis

Shipin gongye ke-ji
Ion mobility spectroscopy is a technique to characterize compounds based on the differences in the migration of sample ions under the combined action of electric field and air flow.
Bo SI, Yating GU, Chen YANG, Yongling LU, Lishuang LÜ   +4 more
doaj   +1 more source

Separation Abilities of Capillary Electrophoresis Coupled with Ion Mobility Mass Spectrometry for the Discrete Detection of Sequence Isomeric Peptides

Separations, 2022
The separation and discrete detection of isomeric sequence peptides with similar properties are important tasks for analytical science. Three different peptide isomers of 12 amino-acid residues long, containing direct and reverse regions of the alanine ...
Yury E. Glazyrin, Gleb G. Mironov, Anna S. Kichkailo, Maxim V. Berezovski   +3 more
doaj   +1 more source

The feeding tube of cyst nematodes:characterisation of protein exclusion [PDF]

, 2014
Plant parasitic nematodes comprise several groups; the most economically damaging of these are the sedentary endoparasites. Sedentary endoparasitic nematodes are obligate biotrophs and modify host root tissue, using a suite of effector proteins, to ...
Jones, John T., Ault James R., John T Jones, Eves-van den Akker, Sebastian, James R. Ault (515791), Alison E Ashcroft, Jones John T., Urwin, Peter E., Jones, JT, Catherine J Lilley, Eves-van den Akker Sebastian, Eves-van den Akker, S, Catherine J. Lilley (182995), Ashcroft, Alison E., Lilley Catherine J., Sebastian Eves-van den Akker, Urwin Peter E., John T. Jones (207247), Ault, James R., James R Ault, Peter E. Urwin (183000), Ashcroft, Alison E, Lilley, Catherine J., Ault, JR, Alison E. Ashcroft (515792), Ashcroft, AE, Ashcroft Alison E., Sebastian Eves-van den Akker (515790), Lilley, CJ, Urwin, PE, Peter E Urwin   +30 more
core   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Ion-shift reagent binding energy and the shift-mass correlation in ion mobility spectrometry [PDF]

, 2022
Ion mobility spectrometry is widely used for the detection of illegal substances and explosives in airports, ports, custom, some stations and many other important places.
Mahmoud, Tabrizchi, Roberto, Fernandez-Maestre, Dairo, Meza-Morelos   +2 more
core   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structure-drift Time Relationships In Ion Mobility Mass Spectrometry

, 2015
Ion mobility spectrometry (IMS) separates ions while they travel through a buffer gas under the influence of an electrical field. The separation is affected by mass and charge but most particularly by shape (collision cross section).
Eberlin M.N., Daroda R.J., Morgon N.H., Pereira R.C.L., Klitzke C.F., Corilo Y.E., de Sa G.F., Heerdt G., Fasciotti M., Lalli P.M., Reis F.A.M., Steffen R.A.   +11 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source