Results 101 to 110 of about 705,503 (291)
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Continuous in vivo measurement of plasma ionized calcium
A new system for continuous in vivo measurement of ionized calcium in blood is presented. The system consists of a small chamber (400 microliter) which is thermostatized at 37 degrees C and contains a glass electrode for pH measurement and a calcium ...
Lang, F. +3 more
core +1 more source
The mixture of pectin and sucrose is gelatinized not only by an acid but also by ionized calcium [PDF]
The mixture of pectin (high methoxylated pectin) and sucrose were gelatinized not only by an acid but by ionized calcium. The gelatinization by calcium was influenced by the concentration of sucrose.
団野, 源一, ダンノ, ゲンイチ
core +1 more source
Background: Dengue fever is a significant pediatric health burden in tropical countries. Predicting severe cases early remains crucial to reducing morbidity and mortality. Hypocalcemia has been implicated in dengue pathogenesis, but its clinical utility
Dr. K Praveen Kumar +3 more
doaj +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The effect of FR167653 on whole-blood ionized calcium levels in PTHrP-treated mice.
PTHrP induces a significant elevation of whole-blood ionized calcium levels in mice within three hours of injection with PTHrP (at day three and five). The whole-blood ionized calcium levels are lower in the FR167653-treated group than in the DW-treated ...
Akira Myoui (326528) +4 more
core +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
In this study, we used an ion-selective membrane electrode to measure ionized calcium in hepatic bile of control +/+ mice and nb/nb mice with hereditary hemolytic anemia.
Kaplan, S A +4 more
core +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source

