Results 51 to 60 of about 1,684 (179)

Diagnostic performance of faecal and tissue multiplex qPCR IS900/F57 for the detection of Mycobacterium avium subspecies paratuberculosis in cattle

Research in Veterinary Science, 2023
Mycobacterium avium subspecies paratuberculosis (MAP) is responsible for bovine-paratuberculosis (bPTB), which causes high production losses in cattle. A cross-sectional study was conducted in 228 cattle to evaluate the validity and diagnostic utility of a multiplex real-time PCR (qPCR) on faecal and intestinal samples [ileocaecal valve (ICV) and ...
Francisco, Jurado-Martos, Fernando, Cardoso-Toset, Carmen, Tarradas, Ángela, Galán-Relaño, José María, Sánchez-Carvajal, Inés, Ruedas-Torres, Eduardo, Vera, Fernanda, Larenas-Muñoz, Lidia, Gómez-Gascón, Irene Magdalena, Rodríguez-Gómez, Librado, Carrasco, Jaime, Gómez-Laguna, Belén Huerta, Lorenzo, Inmaculada, Luque   +13 more
openaire   +4 more sources

Molecular Detection of Mycobacterium avium subsp. paratuberculosis by Targeting IS900 Insertion Sequence in the Samples of Dairy Cattle of Assam, India

Journal of Pure and Applied Microbiology
Johne’s disease (JD), also referred to as paratuberculosis, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). It is a chronic disease of ruminants that primarily affects the enteric system and is seen worldwide.
Nur Abdul Kader, Acheenta G. Barua, Razibuddin Ahmed Hazarika, Premanshu Dandapat, Durlav Prasad Bora, Srinivas Kandhan, Mosuri Chendu Bharat Prasad, Archana Talukdar, Sayed Nabil Abedin, A. Arun Prince Milton, Sandeep Ghatak, Samir Das   +11 more
doaj   +1 more source

New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis

BMC Microbiology, 2006
Background Johne's disease, a serious chronic form of enteritis in ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). As the organism is very slow-growing and fastidious, several PCR-based methods for detection have been developed,
Bölske Göran, Herthnek David
doaj   +1 more source

Application of IS900 PCR for Detection of Mycobacterium avium subsp. paratuberculosis Directly from Raw Milk

Journal of Dairy Science, 2002
A polymerase chain reaction (PCR)-based assay was developed for detection of insertion sequence 900 (IS900) of Mycobacterium avium subsp. paratuberculosis in raw milk. This IS900 PCR assay included DNA extraction and PCR assay using commercially available kits.
S R, Pillai, B M, Jayarao
openaire   +2 more sources

ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. [PDF]

Basrah Journal of Veterinary Research, 2019
Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n  ...
doaj   +1 more source

Detection of Mycobacterium avium subsp. paratuberculosis in an Egyptian mixed breeding farm and comparative molecular characterisation of isolates from cattle, camels and cats – a case report [PDF]

Bulgarian Journal of Veterinary Medicine, 2019
The present study records and investigates an outbreak of Johne’s Disease in a mixed breeding camel – cattle farm and the possible role of non-domestic non-ruminants animals in the epidemiology of Mycobacterium avium subspecies paratuberculosis in Egypt.
M. Salem, W. El-Deeb, K. Abdel-Moein, A. El-Sayed, A. Fayed, F. Housawi, A. Al-Naeem, M. Zschöck   +7 more
doaj   +1 more source

Management of Mycobacterium avium subsp. paratuberculosis in dairy farms: Selection and evaluation of different DNA extraction methods from bovine and buffaloes milk and colostrum for the establishment of a safe colostrum farm bank

MicrobiologyOpen, Volume 8, Issue 10, October 2019., 2019
The aim of this study was to validate different DNA extraction methods for detection Mycobacterium avium subsp. paratuberculosis (MAP) DNA from bovine and buffalo colostrum to support a "safe colostrum farm bank" setup and thus prevent the main within farm MAP transmission route, as well as to allow the MAP free certification of milk products for ...
Fabrizio Gamberale, Gabriele Pietrella, Marcello Sala, Paola Scaramella, Silvia Puccica, Valeria Antognetti, Norma Arrigoni, Matteo Ricchi, Antonella Cersini   +8 more
wiley   +1 more source

Whole-genome analysis of Mycobacterium avium subsp. paratuberculosis IS900 insertions reveals strain type-specific modalities

, 2022
Mycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of Johne’s disease in ruminants. The IS900 insertion sequence (IS) has been used widely as an epidemiological marker and target for PCR diagnosis.
Thierry Cochard (690153), John Bannantine (8498745), Marian Price-Carter (2412403), Maxime Branger (6662270), Richard Whittington (6587144), Cyril Conde (9218690), Franck Biet (30865), Karen Stevenson (1399351)   +7 more
core  

Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods [PDF]

Veterinary World, 2014
Background: Johne's disease (JD) caused by Mycobacterium avium subsp. paratuberculosis (MAP) represents a real threat to the agriculture and dairy food industries and believed to be a potential public health problem.
G. S. Abdellrazeq, M. M. El-Naggar, S. A. Khaliel , A. E. Gamal-Eldin   +3 more
doaj   +1 more source

Evaluating the Effects of 60°C Heating for 90 Min on Bacterial Pathogen Viability and IgG Concentration in Bovine Colostrum

Veterinary Medicine and Science, Volume 11, Issue 4, July 2025.
Heating bovine colostrum at 60°C for up to 90 min eliminates key pathogens, including Escherichia coli, Salmonella Enteritidis, Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium, while preserving IgG levels. This protocol ensures microbial safety and colostrum quality, offering a practical solution for dairy herd health ...
Mostafa Moazeni, Aria Rasooli, Mohammad Nouri, Masoud Ghorbanpoor, Nader Mosavari, Amir Zakian   +5 more
wiley   +1 more source