Results 101 to 110 of about 29,170 (153)
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Isoelectric focusing in polyacrylamide-agarose
Analytical Biochemistry, 1984A procedure using a 2.5% acrylamide-0.5% agarose gel for slab or tube isoelectric focusing is described. This composite gel is durable and enables a rapid focusing of high-molecular-weight compounds.
Richard M Pino, R M Pino
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ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS
Lancet, The, 1968A L Latner, A L Latner
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Journal of Chromatography A, 1974
Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Abstract This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media. We have tried to cover new developments over the years 1972, 1973 and early 1974 since the previous years have already been reviewed. An introductory chapter on the properties and detection of Ampholines is followedby a section on analytical
P G, Righetti, J W, Drysdale
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Isoelectric focusing in agarose
Journal of Immunological Methods, 1979We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused.
C A, Saravis, N, Zamcheck
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Methods of isoelectric focusing
American Journal of Physical Anthropology, 1979AbstractThe method of isoelectric focusing has been avoided by many workers because of expense, technical difficulty, and problems of interpretation. Inexpensive, easy, and interpretable results are possible using equipment and reagents commonly available. Methods which allow these results are presented and explained.
R H, Byles, W, McGuire, M F, Sanders
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Isoelectric focusing of neuraminidase
Experientia, 1970Die isoelektrischen Punkte der Neuraminidasen vonVibrio cholerae (pH 4.80) undClostridium perfringens (pH 4.95) wurden mittels isoelektrischer Fokusierung bestimmt. Neuraminidasen zwei verschiedener Influenzaviren (A2/Aichi/68 und B/Mass/66) wurden entsprechend analysiert.
A R, Neurath, R W, Hartzell, B A, Rubin
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Parallel isoelectric focusing chip
PROTEOMICS, 2004AbstractFast isoelectric focusing (IEF) is becoming a key method in modern protein analysis. We report here the theory and experimental results of new parallel isoelectric devices (PID) for fast IEF. The main separation tool of any PID is a dielectric membrane with conducting channels filled by immobiline gels of varying pH.
Gleb, Zilberstein +3 more
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Improvements of isoelectric focusing in agarose for direct tissue isoelectric focusing
ELECTROPHORESIS, 1982AbstractImprovements of isoelectric focusing (IEF) in agarose are described that result in consistently obtaining broad, linear pH gradients in the range pH 4 to 9.2 at equilibrium. Cathodic drift has been reduced by the use of a blend of commercial carrier ampholytes and by incorporating lysine and arginine in the catholyte as spacers.
Basil J. Thompson +3 more
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Preparative isoelectric focusing in agarose
Analytical Biochemistry, 1981Abstract The use of agarose gels as supporting media for flat-bed preparative isoelectric focusing was applied to the fractionation of serum proteins in the pH range 3.5–6, and red cell hemolysates in the pH range 3–8. The agarose gels are easy to prepare, give linear pH gradients, and do not appear to produce molecular sieving effects.
C, Chapuis-Cellier, P, Arnaud
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