Results 111 to 120 of about 60,313 (296)

Electrophoretic separation of proteins via complexation with a polyelectrolyte

, 2002
We suggest to augment standard isoelectronic focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE).
B.I. Shklovskii, Blatter, E.M. Baskin, G.V. Zilberstein, Grosberg, Kabanov, Netz, Nguyen, Rabilloud, Righetti, Righetti, Shklovskii, Shklovskii   +12 more
core   +1 more source

Secretion of the arginine-rich and A-I apolipoproteins by the isolated perfused rat liver

Journal of Lipid Research, 1977
Rates of secretion of the arginine-rich and A-I apolipoproteins into perfusates of rat livers were measured by specific radioimmunoassays. Livers were perfused for 6 hr in a recirculating system in the presence or absence of 5,5′-dithionitrobenzoic acid,
T E Felker, M Fainaru, R L Hamilton, R J Havel   +3 more
doaj   +1 more source

Detection of a Distinct Erythropoietin (EPO) Profile After Isoelectric Focusing in Patients With Familial Erythrocytosis. [PDF]

Drug Test Anal
A basic EPO IEF profile was identified in patients with familial erythropoiesis linked to mutations in noncoding parts of EPO gene. Comparison of blood and urine EPO profiles from these patients differed from adult wild‐type EPO (WT) and from Eprex‐administered patients. Patients' EPO do not differ from WT EPO after analysis using SDS‐PAGE/SAR‐PAGE. It
Martin L, Idriss S, Maaziz N, Huerre S, Mekacher LR, Lahmek K, Girodon F, Gardie B, Ericsson M, Marchand A.   +9 more
europepmc   +2 more sources

Determination of creatinine and creatine by capillary electrophoresis : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University [PDF]

, 1998
The assessment of creatinine and creatine in biological fluids is important in the evaluation of renal and muscular functions. For routine creatinine determinations in the clinical laboratory, the most frequently used method is the spectrophotometric one
Guo, Hong
core  

Incorporation of Novel Synthetic Glycolipids in Liposomal Nanoparticles Affects Opsonization and In Vivo Clearance

Angewandte Chemie International Edition, EarlyView.
We prepared five glycosylated liposomal nanoparticles (G‐LNPs) to investigate the role of glycosylation and protein corona in modulating the in vivo behavior of G‐LNPs. We show that IgG and complement C3 adsorption enhanced liposomal nanoparticle clearance, with IgG promoting subsequent C3 binding.
Yingjie Yu, Xuehan Li, Yu Gao, Shijia Tao, Xiaofei Li, Lemei Zhao, Wenshuai Han, Hao Fan, Ying Qiu, Man Wang, Luying Zhou, Xiaoyan Fang, Wenhua Yang, Haiyang Zhang, Volker Mailänder, Daniel Crespy, Katharina Landfester, Shuai Jiang, Xiangzhao Mao   +18 more
wiley   +1 more source

Multimodal Chromatography in the Downstream Processing of mAb‐Based Products: Mechanisms, Strategies, and Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Multimodal chromatography has emerged as a powerful tool for the purification of monoclonal antibodies (mAbs) and their derivatives—including antibody fragments (Fabs), Fc‐fusions, bispecific (BsAb), and antibody–drug conjugates (ADCs)—offering enhanced selectivity through the integration of ionic, hydrophobic, hydrogen‐bonding, and π–π ...
Amin Javidanbardan, Marco P․ C․ Marques, Ana M. Azevedo, Daniel G. Bracewell, Stephen Goldrick   +4 more
wiley   +1 more source

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Secretory immunoglobulin A (sIgA) is a promising emerging biopharmaceutical candidate, but it currently lacks a standardized platform for purification. To address this, a novel affinity chromatography resin was developed by immobilizing protein variants of the Streptococcus pneumoniae surface protein, SpsA, as an affinity ligand.
David Scheich, Andres Männik, Oliver Meikar, Joan Teyra, Laura Kibena, Sebastiaan Eeltink, Rainer Hahn, Alois Jungbauer, Nico Lingg   +8 more
wiley   +1 more source

Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process

Biotechnology Progress, EarlyView.
Abstract The growing demand for cost‐efficient and flexible biomanufacturing has increased interest in process‐intensified downstream platforms. This study evaluates an intensified monoclonal antibody (mAb) purification sequence, where two unit operations traditionally performed in batch mode—Protein A capture and low pH virus inactivation (VI)—are ...
Mario Grünberg, Lisa Lipski, Gabriel Fisicaro, Thomas Duvignau, Karolina Meyer‐Heinrichs, Diana Carmen Mocsy, Thomas‐Josef Filz, Bastian Quaas, Alexandra Stützer   +8 more
wiley   +1 more source

Keggin‐Type Aluminum Polyoxometalate‐Mediated Oxidation of Amorphous Carbon for Engineered Electrochemical Interfaces

Carbon Energy, EarlyView.
Keggin‐type Al‐POM‐coated silica achieves selective surface oxidation of amorphous carbon through electrostatic attraction and proton‐coupled oxidation, tailoring interfacial properties for lithium‐ion batteries and semiconductor processes. ABSTRACT Amorphous carbon is widely used in energy storage and semiconductor technologies, where surface ...
Ganggyu Lee, Dongmin Kang, Sumin Hong, Hongjun Park, Myungju Woo, Woojin Jeong, Giha Lee, Junsang Lee, Sangkyun Kim, Jea‐Gun Park, Takamasa Mori, Yehwan Kim, Taeseup Song, Ungyu Paik   +13 more
wiley   +1 more source

Isolation and Purification of Heterotetrameric Catalase from a Desiccation Tolerant Cyanobacterium Lyngbya arboricola [PDF]

Journal of Stress Physiology & Biochemistry, 2013
The desiccation tolerant cyanobacterium Lyngbya arboricola, isolated from bark surfaces of Mangifera indica, possessed up to four stable isoforms of catalase in addition to other antioxidative enzymes, for several years under a dry state. Purification of
Kapoor, Shivali, Tripathi, S. N., Shrivastava, Alpana   +2 more
doaj