Results 131 to 140 of about 35,027 (292)
Вестник защиты растенийThe Sunn pest Eurygaster integriceps and related wheat bugs (Sunn bugs) cause great damage to the wheat crop by injecting hydrolytic enzymes into the grain that liquefy the endosperm during extraintestinal digestion.
А. В. Конарев +1 more
doaj +1 more source
Robustness and Ruggedness of Isoelectric Focusing and Superficially Porous Liquid Chromatography with Fourier Transform Mass Spectrometry. [PDF]
J Am Soc Mass Spectrom, 2021 Corbett JR, Robinson DE, Patrie SM.
europepmc +1 more source
Fractionation of carrier ampholytes for isoelectric focusing
, 1975 A simple method for fractionating synthetic carrier ampholytes is reported, based on the principle of continuous-flow isoelectric focusing in gel-stabilized layers. An 8% ampholyte solution, encompassing the pH range 3-9.5, is separated into 12 fractions
P. G. Righetti +3 more
core +1 more source
PROTEOMICS, EarlyView.ABSTRACT Comprehensive characterization of proteoforms in complex biological systems remains a significant challenge. While native ion‐exchange chromatography (IEC) hyphenated with native mass spectrometry (nMS) is a powerful tool for resolving proteoforms, three critical limitations persist: restricted volatile buffers causing nonlinear pH gradients ...
Ziran Zhai +5 more
wiley +1 more source
, 2016 Preparative (solution) isoelectric focusing (sIEF) is a proven technique for proteome prefractionation, but carries limitations which include the risk of protein loss from isoelectric precipitation, poor focusing, and excessively long separation times ...
John C. Tran (1471192) +1 more
core +1 more source
PROTEOMICS, EarlyView.ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
wiley +1 more source
In Silico Structure‐Guided Design of Peptide Candidates Targeting γ‐Secretase Subunit Assembly
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT The γ‐secretase complex is a membrane‐embedded protease essential for intramembrane cleavage of substrates such as Notch receptors and the amyloid precursor protein (APP), processes central to cancer progression and Alzheimer's disease (AD) pathology.
Selcen Arı Yuka +2 more
wiley +1 more source
Isoelectric focusing of alpha-L-fucosidase from human embryonal carcinoma.
, 1986 Isoelectric focusing profiles of alpha-L-fucosidase recovered from human embryonal carcinoma (EC) and seminoma were compared with those of mouse germ cell tumor-derived stem cells and some human somatic cell neoplasms in an attempt to determine whether ...
Damjanov, I +2 more
core
Structural Insights Into the Function of Leishmania major Adenylosuccinate Lyase
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT One of several intriguing aspects of kinetoplastid biochemistry is the complete dependence on host purines and purine recycling due to the lack of a de novo purine biosynthesis pathway. Adenylosuccinate lyase (ASL, EC 4.3.2.2) is a key enzyme in the purine synthesis pathway responsible for the conversion of adenylosuccinate into adenosine ...
Ivan R. e Silva +10 more
wiley +1 more source
Radioimmunofixation of human ferritin following serum isoelectric focusing.
, 1988 International audienceRadioimmunofixation of human ferritin following isoelectric focusing of serum was developed to study the microheterogeneity of this protein in native serum without previous purification or concentration.
Lasne, Y. +3 more
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