Results 71 to 80 of about 35,027 (292)

Miniaturized isoelectric focusing of proteins and peptides in microchip under gradient electric field

open access: yes, 2007
Isoelectric focusing separation has been regarded as a high-resolution separation technique for amphoteric analytes,such as proteins and ...
张玉奎   +3 more
core  

Isoelectric Focusing in Space

open access: yes, 1981
The potential of space electrophoresis is conditioned by the fact that all electrophoretic techniques require the suppression of gravity-caused convection.
R. A. Mosher   +3 more
core   +1 more source

AS‐pHopt: An Optimal pH Prediction Model Enhanced by Active Site of Enzymes

open access: yesAdvanced Intelligent Discovery, EarlyView.
To address the low accuracy of enzyme optimal pH (pHopt) prediction, this study develops active site‐based pHopt (AS‐pHopt), a prediction model enhanced by active site information and pseudo‐label prediction. Integrating key structural and physicochemical features affecting enzyme pHopt, AS‐pHopt uses Evolutionary Scale Modeling (ESM)‐2 with active ...
Wenxiang Song   +6 more
wiley   +1 more source

Characterization of human very low density lipoproteins containing two electrophoretic populations: double pre-beta lipoproteinemia and primary dysbetalipoproteinemia

open access: yesJournal of Lipid Research, 1977
Two discrete populations of very low density lipoproteins, with fast and slow pre-beta electrophoretic mobility, were found in 50% of normolipemic and 30% of hyperlipemic individuals selected at random.
A Pagnan, Dr   +3 more
doaj   +1 more source

Tensile Stimulation in Biorelevant Culture Conditions Enhances MSC and TPSC Tenogenesis on Aligned Electrospun Scaffolds

open access: yesAdvanced NanoBiomed Research, EarlyView.
Biomimetic electrospun scaffold incorporating GDF‐7‐loaded mesoporous silica nanoparticles, combined with mechanical stimulation and physiological oxygen tension, guides tenogenic differentiation of mesenchymal stromal cell and tendon progenitor stem cell. This integrated approach enhances cell proliferation, matrix deposition, and tendon‐specific gene
Vera Citro   +3 more
wiley   +1 more source

Plasminogen Activator from Porcine Heart Tissue

open access: yesCHIMIA, 1976
By ion exchange chromatography, precipitation with (NH4)2SO4 and gel filtration highly enriched tissue activator of plasminogen is prepared from pig heart tissue. Further purification can be achieved by isoelectric focusing. With the same technique five
P. Cuendet, E.E. Rickli
doaj   +1 more source

Isoelectric focusing and electrophoresis at subzero temperatures

open access: yes, 1978
Electrophoretic and isoelectric focusing separations have been achieved at -20 to -30°C, i.e., at temperatures considerably lower than previously reported by using as supporting media gels of acrylamide-methylacrylate copolymers and dimenthylsulfoxide ...
A. Heyda   +3 more
core   +1 more source

Bovine T cells recognize antigen in association with MHC class II haplotypes defined by one-dimensional isoelectric focusing

open access: yes, 1991
A recently established, one-dimensional isoelectric focusing (IEF) method for distinguishing major histocompatibility complex (MHC) class II polymorphisms in an outbred species, cattle, has allowed us to analyse the involvement of the MHC in the ...
GLASS, EJ, OLIVER, RA, SPOONER, RL
core  

Analysis of genetic variants of transferrin in human serum after desialylation by capillary zone electrophoresis and capillary isoelectric focusing

open access: yes, 2017
Capillary electrophoresis analysis of transferrin in human serum is used to assess genetic variants after desialylation with neuraminidase and iron saturation to reduce the complexity of the transferrin pattern and thus facilitate the recognition of ...
Burda, Patricie   +10 more
core   +1 more source

IEF-2DE Analysis and Protein Identification

open access: yesBio-Protocol, 2013
Isoelectric focusing followed by SDS-PAGE (IEF-2DE) separates proteins in a two-dimensional matrix of protein pI (Protein Isoelectric Point) and molecular weight (MW).
Xia Wu   +3 more
doaj   +1 more source

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