Results 51 to 60 of about 60,313 (296)

Rotating apparatus for isoelectric focusing [PDF]

, 1986
This disclosure is directed to an isoelectric focusing apparatus, wherein stabilization of the fluid containing the isolated proteins is achieved by carrying out the separation in a rotating cylinder with the separation cavity of the cylinder being ...
Bier, Milan
core   +1 more source

The Terminal Domains of Collagen‐Like Silk Direct Molecular Interactions and Liquid–Liquid Phase Separation‐Mediated Material Assembly

Advanced Functional Materials, EarlyView.
Terminal domains of recombinant collagen‐like silk regulate hierarchical self‐assembly across molecular and material scales. We show that terminal domains of collagen‐like silk enhance triple‐helix stability and drive liquid–liquid phase separation, enabling reversible formation of fibers and self‐healing films.
Mengjie Shen, Daniil Astapov, Nur Alia Oktaviani, Mami Goto, Keiji Numata, A. Sesilja Aranko   +5 more
wiley   +1 more source

A new paradigm for optimized experimental design in cIEF platforms aimed at an accurate robust and reliable mAbs charge-variant assessment

Scientific Reports
The success of therapeutic monoclonal antibodies (mAbs) and their biosimilars, highlights the challenge to control their purity, identity, and stability.
Marcello Belfiore, Alessandro Ascione, Virginia Ghizzani, Sabrina Di Meo, Francesca Luciani   +4 more
doaj   +1 more source

Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis

International Journal of Horticultural Science, 2005
Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus.
A. Pedryc, J. Halász, R. Hermán, A. Hegedűs   +3 more
doaj   +1 more source

Identification of ferredoxin II as a major calcium binding protein in the nitrogen-fixing symbiotic bacterium Mesorhizobium loti [PDF]

, 2015
BACKGROUND: Legumes establish with rhizobial bacteria a nitrogen-fixing symbiosis which is of the utmost importance for both plant nutrition and a sustainable agriculture.
Damiani, Ernesto, Ercolin, Flavia, Moscatiello, Roberto, Navazio, Lorella, Roveri, Antonella, Squartini, Andrea, Zaccarin, Mattia   +6 more
core   +1 more source

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Copurification of actin and desmin from chicken smooth muscle and their copolymerization in vitro to intermediate filaments [PDF]

, 1979
Desmin is a 50,000-mol wt protein that is enriched along with 100-A filaments in chicken gizzard that has been extracted with 1 M KI. Although 1 M KI removes most of the actin from gizzard, a small fraction of this protein remains persistently insoluble,
Hubbard, Bruce D., Lazarides, Elias
core   +3 more sources

Engineered Protein‐Based Ionic Conductors for Sustainable Energy Storage Applications

Advanced Materials, EarlyView.
Rational incorporation of charged residues into an engineered, self‐assembling protein scaffold yields solid‐state protein films with outstanding ionic conductivity. Salt‐doping further enhances conductivity, an effect amplified in the engineered variants. These properties enable the material integration into an efficient supercapacitor.
Juan David Cortés‐Ossa, Paolo Blesio, Marcial Fernandez‐Castro, Lisa Almonte, Maxence Fernandez, Mantas Liutkus, Perumal Pandurangan, Carlos Sabater, Aitor Villaverde, Manuel Melle‐Franco, Nurit Ashkenasy, Felipe Jiménez‐Ángeles, M. Carmen Morant‐Miñana, M. Reyes Calvo, Aitziber L. Cortajarena   +14 more
wiley   +1 more source

The Validation of the sickle scan technique used to diagnose sickle cell disease

Orapuh Journal
Introduction Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese ...
Magain Mihatano Barihuta, Guelord Inkalaba, Jocelyn Kakumba Mankulu , Olivier Nzingula, Maguy Luvandu Mbenza, Nicole M. Misengabu, M Ndeme, Michel Diyi, Mbembe Bitengeli, Tresor Bayebila Menanzambi, Nsimba Miezi, José Lami Nzunzu, Fridolin Kodondi Kule-koto   +12 more
doaj   +1 more source

Genetic purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing [PDF]

Crop Breeding and Applied Biotechnology, 2013
Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds.In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line,respectively. Based
Min Yan
doaj