Results 51 to 60 of about 35,027 (292)

The Validation of the sickle scan technique used to diagnose sickle cell disease

Orapuh Journal
Introduction Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese ...
Magain Mihatano Barihuta, Guelord Inkalaba, Jocelyn Kakumba Mankulu , Olivier Nzingula, Maguy Luvandu Mbenza, Nicole M. Misengabu, M Ndeme, Michel Diyi, Mbembe Bitengeli, Tresor Bayebila Menanzambi, Nsimba Miezi, José Lami Nzunzu, Fridolin Kodondi Kule-koto   +12 more
doaj   +1 more source

Genetic purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing [PDF]

Crop Breeding and Applied Biotechnology, 2013
Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds.In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line,respectively. Based
Min Yan
doaj  

αS1-casein in goat milk: identification of genetic variants by Capillary Zone Electrophoresis compared to Isoelectric Focusing

Italian Journal of Animal Science, 2011
AlphaS1 casein fraction in caprine milk is characterized by an important polymorphism due to substitution, deletion of amino acids and post trascriptional modifications (Grosclaude et al., 1994; Ferranti et al., 1997).
G. Enne, M. Feligni, A. Brambilla
doaj   +1 more source

ProSiteHunter: A Unified Framework for Sequence‐Based Prediction of Protein‐Nucleic Acid and Protein‐Protein Binding Sites

Advanced Science, EarlyView.
This study proposed a unified sequence‐based framework for protein binding site prediction, which adopted a tri‐track semantic multi‐source feature fusion strategy to effectively capture diverse macromolecular interaction sites and further improved the accuracy of antibody‐antigen interaction prediction.
Dongliang Hou, Qihang Zhen, Zexin Lv, Xinyue Cui, Suhui Wang, Minghua Hou, Zhan Zhou, Xiaogen Zhou, Guijun Zhang   +8 more
wiley   +1 more source

Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation

, 2009
Monolithic immobilized pH gradient (M-IPG) materials were prepared in microchannles by photoinitiated polymerization of acrylamide, glycidylmethacrylate and Bis, followed by the attachment of focused Ampholine onto the surface of porous monoliths via ...
Liang, Yu, Liang, Zhen, Cong, Yongzheng, Zhang, Yukui, Zhang, Lihua, 张丽华   +5 more
core   +1 more source

Mechanistic Understanding of Protein–MOF Integration Through Surfactant‐Driven Interfacial Design

Advanced Science, EarlyView.
This study reveals how surfactant‐driven interfacial design governs the assembly and stability of protein@MOF composites. Using lipid‐based nonionic surfactants, we modulate protein–MOF interactions to improve encapsulation efficiency, MOF crystallization, and catalytic performance.
Ehsan Rashidniyaghi, Mohammad Khavani, Carlie Coerver, Ruibin Liang, Raheleh Ravanfar   +4 more
wiley   +1 more source

Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing.

Journal of Lipid Research, 1978
ApoC-II and apoC-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea.
A L Catapano, R L Jackson, E B Gilliam, A M Gotto, Jr, L C Smith   +4 more
doaj   +1 more source

TDP‐43 Aggregation: The Healthy‐Toxic Balance of the Prion‐Like Domain

Advanced Science, EarlyView.
TDP‐43 function relies on a delicate balance between reversible phase‐separated states and irreversible aggregation. Under physiological conditions, TDP‐43 forms dynamic droplets and oligomers that support normal cellular functions. In pathological contexts, this balance shifts toward aberrant aggregation, leading to toxic species.
Luca Zangrando, Emanuele Buratti, Francesca Paron   +2 more
wiley   +1 more source

Proteomic analysis of subarachnoid hemorrhage - liquid phase isoelectric focusing in complex protein sample

Journal of Medical Science, 2016
Background: The aim of this study was to present the proteomic approach based on liquid phase isoelectric focusing fractionation coupled to nLC-MALDI-TOF/TOF-MS/MS analysis to characterize cerebrospinal fluid from control patients and those suffering ...
Joanna Hajduk, Bartosz Sokół, Agata Swiatly, Jan Matysiak, Piotr Nowicki, Ewa Garbiec, Norbert Wąsik, Roman Jankowski, Zenon J. Kokot   +8 more
doaj   +1 more source

Isoelectric Focusing of Cassava Protoplasts [PDF]

Plant Physiology, 1991
Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts.
M A, Santana, L, Villegas
openaire   +2 more sources