Results 41 to 50 of about 35,027 (292)

Characterization of a novel Entamoeba histolytica strain from Burkina Faso, Africa, possessing a unique hexokinase-2 gene

Parasite, 2011
An Entamoeba histolytica strain (BF-841 cl1) that originated from Burkina Faso, Africa presented with novel, polymorphic genotypes of the serine-rich E.
Suzuki J., Kobayashi S., Imada M., Tolba M.E., Takeuchi T.   +4 more
doaj   +1 more source

A Piezo‐Mimetic Ionic Hydrogel Harnessing Joint Motion for Cartilage Repair

Advanced Science, EarlyView.
A piezo‐mimetic ionic hydrogel converts mechanical deformation into bioactive ionic currents via stress‐induced asymmetric ion migration. Coupled with sustained Mg2+ release, this mechano‐electrochemical platform bridges mechanical loading and cellular signaling, enabling load‐adaptive stimulation and enhanced cartilage regeneration. ABSTRACT Articular
Chenyuan Gao, Xinyu Wang, Zhifeng Wu, Xinya Zhang, Xiao Geng, Ti Zhang, Yue Wang, Hongwei Xia, Jun Zhang, Xianbo Qiu, Cuiru Sun, Yingjie Yu, Wenli Dai, Hua Tian, Qing Cai   +14 more
wiley   +1 more source

Incorporation of Novel Synthetic Glycolipids in Liposomal Nanoparticles Affects Opsonization and In Vivo Clearance

Angewandte Chemie, EarlyView.
We prepared five glycosylated liposomal nanoparticles (G‐LNPs) to investigate the role of glycosylation and protein corona in modulating the in vivo behavior of G‐LNPs. We show that IgG and complement C3 adsorption enhanced liposomal nanoparticle clearance, with IgG promoting subsequent C3 binding.
Yingjie Yu, Xuehan Li, Yu Gao, Shijia Tao, Xiaofei Li, Lemei Zhao, Wenshuai Han, Hao Fan, Ying Qiu, Man Wang, Luying Zhou, Xiaoyan Fang, Wenhua Yang, Haiyang Zhang, Volker Mailänder, Daniel Crespy, Katharina Landfester, Shuai Jiang, Xiangzhao Mao   +18 more
wiley   +2 more sources

A new paradigm for optimized experimental design in cIEF platforms aimed at an accurate robust and reliable mAbs charge-variant assessment

Scientific Reports
The success of therapeutic monoclonal antibodies (mAbs) and their biosimilars, highlights the challenge to control their purity, identity, and stability.
Marcello Belfiore, Alessandro Ascione, Virginia Ghizzani, Sabrina Di Meo, Francesca Luciani   +4 more
doaj   +1 more source

Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis

International Journal of Horticultural Science, 2005
Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus.
A. Pedryc, J. Halász, R. Hermán, A. Hegedűs   +3 more
doaj   +1 more source

Isoelectric focusing of mycoplasma proteins [PDF]

Applied and Environmental Microbiology, 1976
Polyacrylamide gel isoelectric focusing (PAGIF) in thin layer was used to resolve proteins of Mycoplasma spp., Acholeplasma spp., and eight strains of Ureaplasma urealyticum (T-strain). A mixture of urea, Triton X-100, and dithioerythritol was used to solubilize sonically disrupted cells. PAGIF was performed in the range of pH 3 to 10. Protein patterns
I A, Sayed, B A, Hatten
openaire   +2 more sources

Balancing Stealth and Targetability: cCPP‐ and PEG‐Modified Liposomes for the Targeted Delivery of Anti‐HER2 Nanobodies

Advanced Science, EarlyView.
This study addresses the rapid renal clearance of anti‐HER2 nanobodies by developing specialized liposomal carriers. It details the formulation of dual modified liposomes, demonstrating how PEGylation preserves structural integrity and cell‐penetrating peptides enhance cellular interaction.
Giulia Pander, Maria del Pilar Palacios Cisneros, Clara Certa, Megan Stierli, Katharina Beck, Nicolas Färber, Lisa Blank, Fiona Tanner, Eric Mühlberg, Sabrina Wohlfart, Christian Kleist, Jörg Huwyler, Gert Fricker, Walter Mier, Philipp Uhl   +14 more
wiley   +1 more source

Isoelectric focusing of Chlamydia trachomatis [PDF]

Infection and Immunity, 1979
HeLa-229 cells and the elementary bodies of Chlamydia trachomatis had a net negative electrical surface charge at neutral pH when measured by isoelectric focusing. Inclusion-forming and non-inclusion-forming elementary bodies focused in one band at pI 4.64.
R J, Kraaipoel, A M, van Duin
openaire   +2 more sources

On-line hyphenation of capillary isoelectric focusing and capillary gel electrophoresis by a dialysis interface

, 2003
An on-line two-dimensional (2D) capillary electrophoresis (CE) system consisting of capillary isoelectric focusing (CIEF) and capillary gel electrophoresis (CGE) was introduced. To validate this 2D system, a dialysis interface was developed by mounting a
Yang, C, Zhang, WB, Yang, Q, Zhang, LY, Zhang, YK, Liu, HC   +5 more
core   +1 more source

De Novo Design of Membrane‐Targeting Antimicrobial Peptides Against Gram‐Negative Bacteria Using a Generative Artificial Intelligence Framework

Advanced Science, EarlyView.
Antimicrobial resistance caused by Gram‐negative bacteria remains difficult to overcome due to the protective outer membrane. To address this challenge, a multi‐condition constrained generative AI framework, GenMTAMP is proposed for de novo membrane‐targeting antimicrobial peptide design by integrating physicochemical and spatial structure descriptors.
Jingxiao Yu, Da‐Wen Sun, QingYi Wei, Yibing Zhang, Jidong Tang, Hongbin Pu   +5 more
wiley   +1 more source