Results 21 to 30 of about 35,027 (292)

Evaluation of semi-automatic image analysis tools for cerebrospinal fluid electrophoresis of IgG oligoclonal bands

Practical Laboratory Medicine, 2018
Background: IgG concentrations in cerebrospinal fluid generally range from 20 to 45 mg/L. In multiple sclerosis immune reactions lead to intrathecal synthesis of specific IgGs that can be detected in biological fluid samples both quantitatively and ...
G. Forzy, L. Peyrodie, S. Boudet, Z. Wang, A. Vinclair, V. Chieux   +5 more
doaj   +1 more source

Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting

Biomedical Papers, 2018
Background and Aims: Detection of oligoclonal IgG (o-IgG) in the cerebrospinal fluid (CSF) not found in serum is the principal laboratory test to support a diagnosis of multiple sclerosis.
Ondrej Dlouhy, Pavlina Kusnierova, Irena Kurasova, Monika Cisarikova, David Zeman   +4 more
doaj   +1 more source

Microfluidic high-resolution free-flow isoelectric focusing [PDF]

, 2007
A microfluidic free-flow isoelectric focusing glass chip for separation of proteins is described. Free-flow isoelectric focusing is demonstrated with a set of fluorescent standards covering a wide range of isoelectric points from pH 3 to 10 as well as ...
Kohlheyer, Dietrich, Schasfoort, Richard B.M., Schlautmann, Stefan, van den Berg, Albert, Eijkel, Jan C.T., van den Berg, Albert; id_orcid   +5 more
core   +1 more source

Comparison of pectolytic and proteolytic activities of six clarifying commercial preparations. Incidence of secondary enzymatic activities on proteins in Champagne musts

OENO One, 1996
In order ta estimate c1arifying aptitude and the effects of prefermentation treatment by commercial pectinases, polygalacturonase (PG), pectin Iyase (PL), pectinmethylesterase (PME) and proteolytic activities of six pectinases samples were studied.
Laurent Berthier, Richard Marchal, Alain Maujean   +2 more
doaj   +1 more source

Influence of extraction method on protein profile of soybeans [PDF]

Hemijska Industrija, 2013
Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone ...
Pavlićević Milica Ž., Stanojević Slađana P., Vucelić-Radović Biljana V.   +2 more
doaj   +1 more source

Validation of isoelectric focusing method for recombinant erythropoietin detection in human urine

Saúde & Tecnologia, 2015
Erythropoietin (EPO) is a substance that stimulates red blood cell production, increasing muscle oxygenation and is secreted naturally by the body and excreted in urine in low concentrations.
Ana Sofia Rodrigues Tavares
doaj   +1 more source

Detecting β-Casein Variation in Bovine Milk

Molecules, 2016
In bovine species, β-casein (β-CN) is characterized by genetic polymorphism. The two most common protein variants are β-CN A2 (the original one) and A1, differing from A2 for one amino acid substitution (Pro67 to His67).
Anna Maria Caroli, Salvatore Savino, Omar Bulgari, Eugenio Monti   +3 more
doaj   +1 more source

Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins

, 2005
A new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary.
Yang, C, Zhang, WB, 张玉奎, Zhang, YK, Duan, JC, Zhang, J   +5 more
core   +1 more source

Functional Blood‐Brain Barrier Crossing by Biomimetic M13 Phage Vectors for Targeted Neuronal Delivery

Advanced Healthcare Materials, EarlyView.
This study investigates the M13 bacteriophage as a biomimetic nanovector capable of crossing in vitro models of the blood–brain barrier. By exploiting peculiar transcellular pathways, M13 avoids lysosomal degradation and preserves its structural integrity and functionality.
Silvia Vercellino, Lucia Pappagallo, Francesca De Chirico, Luca Boselli, Hazem Ahmed, Luca Spagnoletti, Giovanni Pittaluga, Paolo Emidio Costantini, Matteo Calvaresi, Pier Paolo Pompa, Alberto Danielli, Fabio Benfenati, Valentina Castagnola   +12 more
wiley   +1 more source

Dynamic Isoelectric Focusing for Proteomics

, 2016
Dynamic isoelectric focusing is a new technique that is related to capillary isoelectric focusing but uses additional high-voltage power supplies to provide control over the shape of the electric field within the capillary.
Luke Tolley (2416234), Robbie Montgomery (2416240), Xuegang Jia (2527960)   +2 more
core   +1 more source