Microchip Isoelectric Focusing Applications
CHIMIA, 2008 This review summarizes all available publications on isoelectric focusing carried out on planar microfabricated devices. Characteristic features of microchip isoelectric focusing (MC-IEF), such as resolution independence of separation length and
Markéta Vlčková, Maria A. Schwarz
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A Mini Review on Capillary Isoelectric Focusing-Mass Spectrometry for Top-Down Proteomics [PDF]
Frontiers in Chemistry, 2021 Mass spectrometry (MS)-based top-down proteomics (TDP) requires high-resolution separation of proteoforms before electrospray ionization (ESI)-MS and tandem mass spectrometry (MS/MS).
Tian Xu, Liangliang Sun
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Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina [PDF]
Bio-Protocol, 2019 Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa +2 more
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Exploring imaged capillary isoelectric focusing parameters for enhanced charge variants quality control [PDF]
Frontiers in ChemistryBiopharmaceuticals are increasingly utilised in the treatment of oncological, inflammatory, and autoimmune diseases, largely due to their exceptional specificity in targeting antigens.
Virginia Ghizzani +5 more
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Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping [PDF]
Practical Laboratory Medicine, 2020 A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also ...
Randa Bittar +7 more
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Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat [PDF]
Journal of Veterinary Research, 2018 Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes.
Różycki Mirosław +4 more
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Identification of plant taxons by isoelectric focusing
International Journal of Horticultural Science, 1999 Differences were demonstrated in esterasei coenzyme pattern of some essential oil producing plants belonging to the Apiaceae family — fennel (Foeniculum vulgare Mill.), angelica (Angelica archangelica L.), lovage (Levisticum officinale Koch.), dill ...
É. Stefanovits-Bányai +5 more
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Isoelectric focusing and isoelectric points of aminoacyl‐tRNA synthetases [PDF]
FEBS Letters, 1983 Isoelectric points and isoelectric focusing behaviour of 10 highly purified eukaryotic aminoacyl‐tRNA synthetases from 3 sources, Saccharomyces cerevisiae, Euglena gracilis and Phaseolus vulgaris were examined. The pI‐values measured on polyacrylamide gels under native conditions are situated between pH 5.0–7.5.
Lorber, B., Giégé, R.
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Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics. [PDF]
PLoS ONE, 2015 Profiling cellular proteome is critical to understanding signal integration during cell fate determination. In this study, the capability of capillary isoelectric focusing (cIEF) immunoassays to detect post-translational modifications (PTM) of protein ...
Mary G Johlfs +4 more
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Isoelectric focusing in a drop. [PDF]
Langmuir, 2011 A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation.
Weiss NG +3 more
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