Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation [PDF]
Scientific Reports, 2017 Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine.
Narendra Padhan +9 more
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Isoelectric Point Separations of Peptides and Proteins
Proteomes, 2017 The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection.
Melissa R. Pergande +1 more
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Multiple sclerosis in Brazil: analysis of cerebrospinal fluid by standard methods [PDF]
Arquivos de Neuro-Psiquiatria, 1999 The demonstration of intrathecal IgG synthesis has been used as an important laboratory parameter to support the diagnosis of multiple sclerosis(MS). The Committee for European Concerted Action for Multiple Sclerosis has recommended a protocol for the ...
MARZIA PUCCIONI-SOHLER +4 more
doaj +1 more source
Reduction and Alkylation of Proteins in 2D Gel Electrophoresis: Before or after Isoelectric Focusing? [PDF]
Frontiers in Chemistry, 2017 Xiaolin Wu, Chenhui Xu, Wei Wang
doaj +2 more sources
Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies [PDF]
, 1993 In biological fluids, hCG and its free alpha- (hCG alpha) and beta-subunits (hCG beta), occur in multiple forms. These various forms differ at the molecular level primarily in glycosylation, but also differ in protein backbone modifications corresponding
Mann, Klaus +9 more
core +1 more source
SUBTYPE DETERMINATION OF PHOSPHOGLUCOMUTASE (PGM) AND GROUP- SPECIFIC COMPONENT (Gc) BY ISOELECTRIC FOCUSING AMONG SOME IRANIAN POPULATIONS [PDF]
Iranian Journal of Public Health, 1996 A total of 781 serum and 827 red cell samples from four populations of Iran (Kashan, Ardestan, Asrara and Ardabil) were sub-typed by isoelectric focusing for group-specific componet and red cell enzyme Phosphoglucomutase, locus one, respectively ...
S.Y. Seyedna
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IEEE Access, 2022 Hemoglobinopathies are inherited red blood cell disorders. They are commonly screened from newborns early after birth, and if not detected early and treated properly, some of them can be fatal.
Santeri Mantyniemi +2 more
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International Journal of Electrochemical Science, 2016 Alpha 1-antitrypsin (AAT) deficiency is onne of the most hereditary disorders in the world, but there is misdiagnosis for this deficiency. There are about 100 different AAT variants cannot be easily detected by the known methods such as genotyping which ...
Noura Al-Jameil +5 more
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Isoelectric Focusing of Parvoviruses
Intervirology, 1978 Adenovirus-associated virus type 4 and X14 migrate during electrophoresis to pH 2.6 in sucrose-stabilized, pH 2.5-6.0 gradients. Naturally occurring empty capsids appear to have the same isoelectric point as complete virus particles.
R J, Salo, H D, Mayor
openaire +2 more sources
Isoelectric focusing of cerebrospinal fluid significance in differential diagnosis of multiple sclerosis [PDF]
Scripta MedicaBackground/Aim: Isoelectric focusing (IEF) of cerebrospinal fluid (CSF) is the gold standard for detecting intrathecal oligoclonal immunoglobulin G (IgG). Besides its diagnostic and predictive significance, the IEF method also has differential diagnostic
Grgić Sanja +4 more
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