Results 31 to 40 of about 60,313 (296)

Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg [PDF]

open access: yes, 2013
While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC ...
Mayr, Manuel, Rabilloud, Thierry
core   +4 more sources

Validation of isoelectric focusing method for recombinant erythropoietin detection in human urine

open access: yesSaúde & Tecnologia, 2015
Erythropoietin (EPO) is a substance that stimulates red blood cell production, increasing muscle oxygenation and is secreted naturally by the body and excreted in urine in low concentrations.
Ana Sofia Rodrigues Tavares
doaj   +1 more source

Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]

open access: yes, 1984
An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH   +2 more
core   +3 more sources

Detecting β-Casein Variation in Bovine Milk

open access: yesMolecules, 2016
In bovine species, β-casein (β-CN) is characterized by genetic polymorphism. The two most common protein variants are β-CN A2 (the original one) and A1, differing from A2 for one amino acid substitution (Pro67 to His67).
Anna Maria Caroli   +3 more
doaj   +1 more source

Structural Analysis of the Effect of Asn107Ser Mutation on Alg13 Activity and Alg13-Alg14 Complex Formation and Expanding the Phenotypic Variability of ALG13-CDG

open access: yesBiomolecules, 2022
Congenital Disorders of Glycosylation (CDG) are multisystemic metabolic disorders showing highly heterogeneous clinical presentation, molecular etiology, and laboratory results.
Karolina Mitusińska   +5 more
doaj   +1 more source

Isolation and characterisation of two chymotrypsins from Allocyttus niger (black oreo dory) viscera [PDF]

open access: yes, 1997
Two serine proteases from the viscera of deep-sea fish, black oreo dory (Allocyttus niger),were purified by hydrophobic, affinity, and cation exchange chromatography.
Daniel, Roy M., Krzyzosiak, J.
core   +2 more sources

Effects of Biological Sex and Age on Cerebrospinal Fluid Markers—A Retrospective Observational Study

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Cerebrospinal fluid (CSF) analysis is a key diagnostic tool for neurological diseases. To date, only a few studies have investigated in larger cohorts the effect of age and biological sex on diagnostic markers extracted from CSF. Methods For this retrospective observational study, 4163 CSF findings (2012–2020) were evaluated.
Isabel‐Sophie Hafer   +3 more
wiley   +1 more source

Highly Sensitive Electrochemical Biosensor Based on Hairy Particles with Controllable High Enzyme Loading and Activity

open access: yesAdvanced Functional Materials, EarlyView.
For the first time, a highly sensitive electrochemical biosensor based on SiO2‐based hairy particles with a grafted PDMAEMA polymer brush containing a quantifiable and large amount of immobilized Laccase is reported. The fabricated biosensor exhibits a sensitivity of 0.14 A·m⁻¹, a limit of detection (LOD) of 0.1 µm, and a detection range of 0.3–750 µm,
Pavel Milkin   +7 more
wiley   +1 more source

The purification and characterisation of novel dipeptidyl peptidase IV-like activity from bovine serum [PDF]

open access: yes, 2004
The discovery of a potentially novel proline-specific peptidase from bovine serum is presented which is capable of cleaving the dipeptidyl peptidase IV (DPIV) substrate Gly-Pro-MCA.
Abbott   +31 more
core   +1 more source

Smart, Bio‐Inspired Polymers and Bio‐Based Molecules Modified by Zwitterionic Motifs to Design Next‐Generation Materials for Medical Applications

open access: yesAdvanced Functional Materials, EarlyView.
Bio‐based and (semi‐)synthetic zwitterion‐modified novel materials and fully synthetic next‐generation alternatives show the importance of material design for different biomedical applications. The zwitterionic character affects the physiochemical behavior of the material and deepens the understanding of chemical interaction mechanisms within the ...
Theresa M. Lutz   +3 more
wiley   +1 more source

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