Results 31 to 40 of about 29,170 (153)

Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis

International Journal of Horticultural Science, 2005
Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus.
A. Pedryc, J. Halász, R. Hermán, A. Hegedűs   +3 more
doaj   +1 more source

Finger printing human norovirus-like particles by capillary isoelectric focusing with whole column imaging detection

Virus Research, 2022
Owing to the limitation of in vitro culture of human noroviruses (HuNoVs), the development of HuNoV vaccines has to depend on the self-assembling virus-like particles (VLPs) with capsid protein expression.
Jialiang Du, Gang Wu, Chunbo Cui, Chuanfei Yu, Yongfei Cui, Luyun Guo, Yueyue Liu, Yan Liu, Wenbo Wang, Chunyu Liu, Zhihao Fu, Meng Li, Sha Guo, Xiaojuan Yu, Yalan Yang, Maoqin Duan, Gangling Xu, Lan Wang   +17 more
doaj   +1 more source

The Validation of the sickle scan technique used to diagnose sickle cell disease

Orapuh Journal
Introduction Sickle cell disease is a multifactorial haemoglobinopathy that causes various abnormalities in affected individuals. Several diagnostic methods exist, but most require sophisticated techniques that are not available in Congolese ...
Magain Mihatano Barihuta, Guelord Inkalaba, Jocelyn Kakumba Mankulu , Olivier Nzingula, Maguy Luvandu Mbenza, Nicole M. Misengabu, M Ndeme, Michel Diyi, Mbembe Bitengeli, Tresor Bayebila Menanzambi, Nsimba Miezi, José Lami Nzunzu, Fridolin Kodondi Kule-koto   +12 more
doaj   +1 more source

Genetic purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing [PDF]

Crop Breeding and Applied Biotechnology, 2013
Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds.In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line,respectively. Based
Min Yan
doaj  

αS1-casein in goat milk: identification of genetic variants by Capillary Zone Electrophoresis compared to Isoelectric Focusing

Italian Journal of Animal Science, 2011
AlphaS1 casein fraction in caprine milk is characterized by an important polymorphism due to substitution, deletion of amino acids and post trascriptional modifications (Grosclaude et al., 1994; Ferranti et al., 1997).
G. Enne, M. Feligni, A. Brambilla
doaj   +1 more source

Isoelectric focusing of mycoplasma proteins [PDF]

Applied and Environmental Microbiology, 1976
Polyacrylamide gel isoelectric focusing (PAGIF) in thin layer was used to resolve proteins of Mycoplasma spp., Acholeplasma spp., and eight strains of Ureaplasma urealyticum (T-strain). A mixture of urea, Triton X-100, and dithioerythritol was used to solubilize sonically disrupted cells. PAGIF was performed in the range of pH 3 to 10. Protein patterns
I A, Sayed, B A, Hatten
openaire   +2 more sources

Isoelectric focusing of Chlamydia trachomatis [PDF]

Infection and Immunity, 1979
HeLa-229 cells and the elementary bodies of Chlamydia trachomatis had a net negative electrical surface charge at neutral pH when measured by isoelectric focusing. Inclusion-forming and non-inclusion-forming elementary bodies focused in one band at pI 4.64.
R J, Kraaipoel, A M, van Duin
openaire   +2 more sources

Quantification of apoC-II and apoC-III of human very low density lipoproteins by analytical isoelectric focusing.

Journal of Lipid Research, 1978
ApoC-II and apoC-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea.
A L Catapano, R L Jackson, E B Gilliam, A M Gotto, Jr, L C Smith   +4 more
doaj   +1 more source

Proteomic analysis of subarachnoid hemorrhage - liquid phase isoelectric focusing in complex protein sample

Journal of Medical Science, 2016
Background: The aim of this study was to present the proteomic approach based on liquid phase isoelectric focusing fractionation coupled to nLC-MALDI-TOF/TOF-MS/MS analysis to characterize cerebrospinal fluid from control patients and those suffering ...
Joanna Hajduk, Bartosz Sokół, Agata Swiatly, Jan Matysiak, Piotr Nowicki, Ewa Garbiec, Norbert Wąsik, Roman Jankowski, Zenon J. Kokot   +8 more
doaj   +1 more source

Results in the determination of some Hosta varieties by the method of isoelectric focusing

International Journal of Horticultural Science, 2001
Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing
A. Mándy, É. Stefanovits-Bányai, Cs. Imre, Zs. Szafián   +3 more
doaj   +1 more source