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Determination of the isoelectric point of proteins by capillary isoelectric focusing
Journal of Chromatography A, 2004Different ways of determining isoelectric points (pI) of proteins in capillary isoelectric focusing are reviewed here. Due to the impossibility of direct pH measurements in the liquid phase, such assessments have to rely on the use of pI markers. Different types of pI markers have been described: dyes, fluorescently labelled peptides, sets of proteins ...
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Isoelectric points and molecular weights of proteins
Journal of Chromatography A, 1976Proteins with known isoelectric points (pI), as determined by isoelectric focusing, have been tabulated. When available, the native molecular weight as well as sub-unit molecular weight and stoichiometry have been reported. For each entry, the source and, when applicable, the organ of origin and/or subcellular location are given.
P G, Righetti, T, Caravaggio
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Cross Partition and Isoelectric Points of Proteins
Nature, 1970AQUEOUS mixtures of dextran and polyethylene glycol give rise to liquid two-phase systems suitable for the partition of proteins1–5. The partition behaviour of proteins depends to a great extent on the ionic composition of the phase system and on the charge of the protein. Negatively charged materials, for example, have higher partition coefficients in
P A, Albertsson, S, Sasakawa, H, Walter
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Electrophoresis, 2003
Synthetic UV-detectable peptide pI markers were used to estimate isoelectric point (pI) values of proteins separated by capillary isoelectric focusing (CIEF) followed by cathodic mobilization in the absence of denaturing agents. The pI calculation and quantitative analysis of purified proteins showed the feasibility of these peptides as pI markers and ...
Ya, Jin +3 more
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Synthetic UV-detectable peptide pI markers were used to estimate isoelectric point (pI) values of proteins separated by capillary isoelectric focusing (CIEF) followed by cathodic mobilization in the absence of denaturing agents. The pI calculation and quantitative analysis of purified proteins showed the feasibility of these peptides as pI markers and ...
Ya, Jin +3 more
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THE ISOELECTRIC POINT OF BACTERIAL CELLS
Journal of Bacteriology, 1953V P, HARDEN, J O, HARRIS
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Isoelectric Point of Glycinin.
The Journal of Physical Chemistry, 1936R. J. Hartman, L. T. Cheng
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