Results 81 to 90 of about 5,260,747 (202)
Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002% to 0.015%.
Salmenkallio, Marjatta, Kauppinen, Veli
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Production of doubled haplois in Brassica purpuraria
Relevance. In recent years vegetable crop Brassica rapa ssp. chinensis var. purpuraria (synonyms: Brassica campestris L. var.purpurea Bailey; Brassica rapa L. ssp. chinensis var.
E. V. Kozar +6 more
doaj +1 more source
Isolated microspore culture technology of triticale ( × Triticosecale Wittmack)
Triticale ( × Triticosecale Wittm.) is a synthetic hybrid of wheat (Triticum) and rye (Secale), it combines valuable traits of both parental species: high feeding value with low growth requirements. To develop and accelerate the breeding of this feed crop in Kazakhstan, the use of such a biotechnological method as androgenic technology. Plants- doubled
openaire +2 more sources
Development of callus colonies from the isolated microspore culture of capsicum annuum l.
Androgenesis in Capsicum annuum L. via isolated microspore culture, with a limited scope, was studied. Donor genotype, i.e. U-130, was a doubled haploid. Intact flower buds containing microspores from early to late uninucleate stages were pretreated with
Abak K. +7 more
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Part I. The use of doubled haploid plants in a wheat breeding program requires an efficient regeneration system. While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated ...
Gustafson, Vicki Diane
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Microspore embryogenesis in almond (Prunus dulcis Mill.)
This chapter describes a protocol to regenerate microspore derived embryo via microspore embryogenesis through in vitro almond anthers ...
Cimò G +3 more
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Anthers and microspores of Apium graveolens L. (celery) were cultured in vitro to obtain haploid plants.Anther culture: Immature anthers of 'Cornell 619' and 'Cornell 19' at three stages of development were cultured on eight different media containing a half strength of Murashige and Skoog (1/2 MS) and B5 media supplemented with combinations of 2, 4-D,
Dohya, Norio +2 more
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Effects of embryo induction media and pretreatments in isolated microspore culture of hexaploid wheat (Triticum aestivum L. cv. Falat) [PDF]
Isolated microspores of many plants can be induced in vitro to switch their developmental process from the gametophytic to a sporophytic pathway under appropriate conditions and produce haploid plants.
Mousavi, A +2 more
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Anther and microspore cultures of barley and wheat
This paper briefly cites the various procedures for the production of doubled haploids in barley and wheat. Various associated terms are defined and the factor involved in haploid production are outlined.
Kasha, Ken J. +5 more
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Comparison of anther and microspore culture in androgenic embryogenesis and regeneration of broccoli (Brassica oleracea L. var. italica P.) [PDF]
The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and ...
Qin, Ying +3 more
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