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Malic Dehydrogenase Isozymes [PDF]
Nature, 1965 IT has been shown previously1–3 that at least two isozymes of malic dehydrogenase are present in the extracts of various tissues. In addition, the distinct characteristics of these isozymes have been demonstrated by the recent reports4–6.
Kozo Yamada +2 more
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Lipoxygenase isozymes of peanut
Lipids, 1975AbstractLipoxygenase was isolated and partially purified from peanut seed by ammonium sulfate precipitation, gel filtration, and ion exchange column chromatography. Three isozymes of lipoxygenase were identified. Two had pH optima of 6.2, and the other an optimum of 8.3. Molecular weight of each isozyme was 7.3×104, as determined by gel filtration. The
Harold E. Pattee +2 more
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BioScience, 1975
Isozymes are now a common part of the scientific vocabulary, but their recognition is relatively recent, having been first announced in 1959 (Markert and Mqller). Prior to that time, molecular heterogeneity had often been noted in enzyme preparations, but such heterogeneity was usually attributed to contaminants or to partially denatured or degraded ...
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Isozymes are now a common part of the scientific vocabulary, but their recognition is relatively recent, having been first announced in 1959 (Markert and Mqller). Prior to that time, molecular heterogeneity had often been noted in enzyme preparations, but such heterogeneity was usually attributed to contaminants or to partially denatured or degraded ...
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Microheterogeneity of Kasahara isozyme
Clinica Chimica Acta, 1986The microheterogeneity of Kasahara isozyme was investigated by affinity electrophoresis with Con A as the affinity ligand in combination with polyacrylamide gradient gel electrophoresis. On two-dimensional Con A-containing agarose gel electrophoresis, the Kasahara isozyme was separated into three molecular species.
Kazuhisa Taketa +8 more
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Urological Research, 1990
We determined concentrations of alpha and gamma-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of alpha-enolase were 4,170 +/- 2,040 ng/mg protein in normal testis (n = 8) and 8,140 +/- 4,480 ng/mg protein in seminoma (n = 8).
Tatsuya Nagai +4 more
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We determined concentrations of alpha and gamma-enolases in normal testis and in seminoma tissues by enzyme immunoassay. Concentrations of alpha-enolase were 4,170 +/- 2,040 ng/mg protein in normal testis (n = 8) and 8,140 +/- 4,480 ng/mg protein in seminoma (n = 8).
Tatsuya Nagai +4 more
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Angewandte Chemie International Edition in English, 1962
Abstract“Heteroenzyme” is the term applied to proteins of different origin that differ in their physical, chemical, and biochemical properties, but have the same biological action. Enzymes that have the same origin, and consist of very similar, but distinguishable proteins have been named “isozymes” [*]; they may also be designated as “multiple forms.”
T. Wieland, G. Pfleiderer
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Abstract“Heteroenzyme” is the term applied to proteins of different origin that differ in their physical, chemical, and biochemical properties, but have the same biological action. Enzymes that have the same origin, and consist of very similar, but distinguishable proteins have been named “isozymes” [*]; they may also be designated as “multiple forms.”
T. Wieland, G. Pfleiderer
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Isozyme Specificity to Temperature
Nature New Biology, 1972TEMPERATURE treatment of styles leads to self-compatibility in otherwise self-incompatible species of Oenothera2,3, Lilium4 and Trifolium5,6. I have studied the temperature treatment of esterase in styles of several species and hybrids of Lilium by using gel electrophoresis.
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Nature, 1964
IT has previously been observed that crystalline salivary amylase gives two peaks in free electrophoresis1. The work recorded here shows that a sample of salivary amylase crystallized three times, which gave a single peak in the ultracentrifuge (Fig. 1), could be separated into four bands by disk electrophoresis on polyacrylamide gel2 (Fig. 2).
Jean M. Vnenchak, Jytte Muus
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IT has previously been observed that crystalline salivary amylase gives two peaks in free electrophoresis1. The work recorded here shows that a sample of salivary amylase crystallized three times, which gave a single peak in the ultracentrifuge (Fig. 1), could be separated into four bands by disk electrophoresis on polyacrylamide gel2 (Fig. 2).
Jean M. Vnenchak, Jytte Muus
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1973
Publisher Summary This chapter focuses on the concept of isozymes. In 1964, a subcommittee of the International Union of Biochemistry recommended that multiple enzyme forms in a single species, catalyzing essentially the same reaction but differing in various ways should be called “isozymes,” or “isoenzymes.” Nevertheless this definition was too ...
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Publisher Summary This chapter focuses on the concept of isozymes. In 1964, a subcommittee of the International Union of Biochemistry recommended that multiple enzyme forms in a single species, catalyzing essentially the same reaction but differing in various ways should be called “isozymes,” or “isoenzymes.” Nevertheless this definition was too ...
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Hypothyroidism and Isozyme Elevations
Archives of Internal Medicine, 1984To the Editor. —In the JanuaryArchives, Klein and Levey 1 discussed unusual manifestations of hypothyroidism. In their discussion of musculoskeletal features, elevations of the levels of creatine kinase (CK) and lactate dehydrogenase (LDH) were mentioned as being observed in up to 80% of the patients with hypothyroidism.
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