Results 131 to 140 of about 321,959 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Solving of a Variational Inequality Problem Under the Presence of Computational Errors

Mathematics
W. Takahashi and M. Toyoda (2003) proved weak convergence of an iteration process of solving a variational inequality problem for an inverse strongly-monotone mapping.
Alexander J. Zaslavski
doaj   +1 more source

Variational iteration algorithm-II for solving linear and non-linear ODEs

, 2012
Variational iteration method is widely used for solving linear and non-linear ODE. In this paper, the variational iteration algorithm-II is constructed and some examples are given to show its simple solution procedure and high accuracy of the obtained ...
Luis Hernández Martínez
core  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Reduplicative coordination in Spanish

Isogloss
The present paper aims to analyze the properties of Spanish reduplicative coordination (ReCo) within a generative framework. Therefore, it intends to systematically contrast Spanish ReCo with the characterization and predictions made for English by De ...
Laura Malena Kornfeld
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

An iterative output feedback controller-design strategy for energy-to-peak structural vibration control

Journal of Asian Architecture and Building Engineering
To address the issue of inadequate control effect resulting from the challenge of measuring state feedback information comprehensively, this paper proposes an iterative output feedback energy-to-peak control method that solely depends on partial external
Qinghu Xu, Xiang Ruan, Jianhao Wei
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The Dynamic String-Averaging Method for Inverse Strongly-Monotone Operators with Summable Errors

Axioms
In the work by W. Takahashi and M. Toyoda (2003) it was introduced and studied an iterative process for solving a variational inequality problem which is induced by a inverse strongly-monotone mapping.
Alexander J. Zaslavski
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source