Results 11 to 20 of about 17,544 (219)
iQuantitator: A tool for protein expression inference using iTRAQ [PDF]
BMC Bioinformatics, 2009 Background Isobaric Tags for Relative and Absolute Quantitation (iTRAQ™) [Applied Biosystems] have seen increased application in differential protein expression analysis.
Comte-Walters Susana +4 more
doaj +6 more sources
Addressing Accuracy and Precision Issues in iTRAQ Quantitation [PDF]
Molecular & Cellular Proteomics, 2010 iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology that allows quantitative comparison of protein abundance by measuring peak intensities of reporter ions released from iTRAQ-tagged peptides by fragmentation during MS/MS.
Karp, Natasha +5 more
openaire +5 more sources
Isoelectric Focusing of iTRAQ-Labeled Yeast
Cold Spring Harbor Protocols, 2011 INTRODUCTIONA commercial reagent, known as the isobaric tag for relative and absolute quantification (iTRAQ), makes it possible to analyze multiple samples simultaneously. The ability of iTRAQ to compare relative protein abundances across as many as eight samples is a significant advantage over other stable isotope strategies, such as stable isotope ...
Eric S. Simon
openaire +3 more sources
Labeling Yeast Peptides with the iTRAQ Reagent
Cold Spring Harbor Protocols, 2011 INTRODUCTIONA commercial reagent, known as the isobaric tag for relative and absolute quantification (iTRAQ), makes it possible to analyze multiple samples simultaneously. The ability of iTRAQ to compare relative protein abundances across as many as eight samples is a significant advantage over other stable isotope strategies, such as stable isotope ...
Eric S. Simon
openaire +3 more sources
, 2012 Quantitative proteomic analysis can help elucidating unexplored biological questions; it, however, relies on highly reproducible experiments and reliable data processing. Among the existing strategies, iTRAQ is known as an easy to use method allowing relative comparison of up to eight multiplexed samples.Once the data is acquired it is important that ...
Marc, Vaudel +4 more
openaire +3 more sources
Electron Transfer Dissociation of iTRAQ Labeled Peptide Ions [PDF]
Journal of Proteome Research, 2008 Triply and doubly charged iTRAQ ( isobaric tagging for relative and absolute quantitation) labeled peptide cations from a tryptic peptide mixture of bovine carbonic anhydrase II were subjected to electron transfer ion/ion reactions to investigate the effect of charge bearing modifications associated with iTRAQ on the fragmentation pattern. It was noted
Hongling, Han +3 more
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Preparation of Peptides from Yeast Cells for iTRAQ Analysis [PDF]
Cold Spring Harbor Protocols, 2011 INTRODUCTIONA commercial reagent, known as the isobaric tag for relative and absolute quantification (iTRAQ), makes it possible to analyze multiple samples simultaneously. The ability of iTRAQ to compare relative protein abundances across as many as eight samples is a significant advantage over other stable isotope strategies, such as stable isotope ...
Eric S. Simon
openaire +3 more sources
Monitoring the Effect of Tributyltin Salicylate and Propionate, Retinoid X Receptor Ligands, on Heat Shock Protein Expression in MDA-MB-231 Cells by MALDI MS/MS. [PDF]
J Mass SpectromABSTRACT Organotin (IV) compounds are known to induce apoptosis via the intrinsic mitochondrial pathway, which is a key mechanism of effective anticancer therapy. Their ability to selectively promote apoptotic cell death highlights their potential as chemotherapeutic agents. In this study, the in vitro effects of two triorganotin compounds, tributyltin
Strouhalova D +3 more
europepmc +2 more sources
Proteomic Biomarker Discovery in Breast Cancer: Advances, Challenges, and Translational Prospects. [PDF]
J Biochem Mol ToxicolABSTRACT Breast cancer remains a leading cause of cancer‐related morbidity and mortality among women worldwide. Despite major progress in genomic classification, molecular pathology, and targeted therapy, the identification of clinically reliable proteomics‐derived biomarkers for early detection, prognostic stratification, treatment response prediction,
Hassani M +8 more
europepmc +2 more sources
The NADPH oxidase NOX4 regulates redox and metabolic homeostasis preventing HCC progression
Hepatology, EarlyView., 2022 Loss of NOX4 in HCC tumor cells induces metabolic reprogramming in a Nrf2/MYC‐dependent manner to promote HCC progression. Abstract Background and Aims The NADPH oxidase NOX4 plays a tumor‐suppressor function in HCC. Silencing NOX4 confers higher proliferative and migratory capacity to HCC cells and increases their in vivo tumorigenic potential in ...
Irene Peñuelas‐Haro +14 more
wiley +1 more source