Results 191 to 200 of about 17,544 (219)

Quantitative Phosphoproteomic Analysis Using iTRAQ Method

2014
The MAPK (mitogen-activated kinase) cascade plays important roles in plant perception of and reaction to developmental and environmental cues. Phosphoproteomics are useful to identify target proteins regulated by MAPK-dependent signaling pathway. Here, we introduce the quantitative phosphoproteomic analysis using a chemical labeling method.
Tomoya, Asano, Takumi, Nishiuchi
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iTRAQ-based quantitative secretome analysis of Phanerochaete chrysosporium

Journal of Proteomics, 2011
The basidiomycete fungi such as Phanerochaete chrysosporium secrete large amount of hydrolytic and oxidative enzymes and degrade lignocellulosic biomass. The lignin depolymerizing proteins were extensively studied, but cellulose, hemicellulose and pectin hydrolyzing enzymes were poorly explored. In this study P.
Arulmani, Manavalan, Sunil S, Adav, Siu Kwan, Sze   +2 more
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iTRAQ: a method to elucidate cellular responses to mycotoxin zearalenone

Journal of Applied Toxicology, 2012
ABSTRACTMycotoxin zearalenone (ZEN) is a secondary metabolite produced by some Fusarium species that contaminate a large variety of grains and feedstuffs worldwide. ZEN has been associated with a wide variety of adverse health effects including hepatotoxic, hematologic, immunotoxic and genotoxic.
Amel Chatti, Gazzah, Luc, Camoin, Salwa, Abid, Hassen, Bacha, Moncef, Ladjimi   +4 more
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Pilot Application of iTRAQ to the Retinal Disease Macular Telangiectasia

Journal of Proteome Research, 2011
We used the comparative proteomic technique iTRAQ coupled with offline 2DLC-MS/MS to analyze a rare specimen of the poorly understood, potentially blinding ophthalmic condition Macular Telangiectasia type 2 (MacTel type 2). We refined the technique using an internal standard consisting of pooled samples for each iTRAQ experiment to allow for multiple ...
Alice C L, Len, Michael B, Powner, Ling, Zhu, Gregory S, Hageman, Xiaomin, Song, Marcus, Fruttiger, Mark C, Gillies   +6 more
openaire   +2 more sources

Robust Workflow for iTRAQ-Based Peptide and Protein Quantification

2012
Quantitative proteomics has become a routinely used technique to globally compare protein content and expression profiles of biological samples, for instance after differential stimulation. In this context, chemical stable isotope-based labeling techniques, such as ICAT and iTRAQ, have been successfully applied in a large variety of studies.
Florian, Beck, Julia Maria, Burkhart, Joerg, Geiger, René Peiman, Zahedi, Albert, Sickmann   +4 more
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iTRAQ‐Based Quantitative Proteomic Analysis of Nasopharyngeal Carcinoma

Journal of Cellular Biochemistry, 2015
ABSTRACTNasopharyngeal carcinoma (NPC) is a common disease in the southern provinces of China with a poor prognosis. To better understand the pathogenesis of NPC and identify proteins involved in NPC carcinogenesis, we applied iTRAQ coupled with two‐dimensional LC‐MS/MS to compare the proteome profiles of NPC tissues and the adjacent non‐tumor tissues.
Xin-Zhang, Cai, Wei-Qun, Zeng, Yi, Xiang, Yi, Liu, Hong-Min, Zhang, Hong, Li, Sha, She, Min, Yang, Kun, Xia, Shi-Fang, Peng   +9 more
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Fungal Secretomics Through iTRAQ-Based Analysis

Our understanding of how fungi respond and adapt to external environments can be increased by the comprehensive data sets of fungal-secreted proteins. Fungi produce a variety of secreted proteins, and environmental conditions can easily influence the fungal secretome.
Lu, Zheng, Ruonan, Wang, Yuchen, Fei, Yilin, Pan, Peijun, Zhou, Renfang, Shen, Ping, Lan   +6 more
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Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders Using iTRAQ

2012
Aberrant protein glycosylation has been recognized to be associated with many neurodegenerative disorders, including Alzheimer and Parkinson disease. Using mass spectrometry-based technologies to catalog and quantify glycoproteins in these diseases is expected to provide insight into not only the biochemical pathogenesis of neurodegeneration but also ...
Min, Shi, Hyejin, Hwang, Jing, Zhang
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iTRAQ定量升级

2018
在细胞，植物，动物及其他类型适合的样品中测试并优化iTRAQ减少组分实验技术，将20组分缩减至10组分，并保持蛋白总鉴定数达到20组分的85%以上，肽段总鉴定数也与20组分相当或更高。定量方面通过内标肽段鉴定及定量效果进行定量准确性评估，达到或超过20组分的定量水平。
openaire   +1 more source

Proteomic Analysis of Posttranslational Modifications Using iTRAQ in Leishmania

2014
iTRAQ is a high coverage quantitative proteomics technique identifies and quantitates abundance changes of multiple (up to eight) distinct protein samples. To date, one iTRAQ-MS/MS assay can identify up to quarter of cells proteome. Each of the eight tags covalently binds to the N-terminus as well as arginine and lysine side chains of peptides ...
openaire   +2 more sources