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Phytogeny of genusGlossina (Diptera:Glossinidae) according to ITS2 sequences
Science in China Series C: Life Sciences, 1999The flies of genusGlossina (Diptera: Glossinidae) are an important vector of African trypanosomiases which cause diseases in humans and animals. The ribosomal DNA Internal Transcribed Spacer-2 (ITS-2) region sequences from differentGlossina species were PCR-amplified and analyzed in order to construct a molecular phylogeny for genusGlossina.
X, Chen, S, Li, C, Li, S, Zhao, A, Serap
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Identification of astigmatid mites using ITS2 and COI regions
Parasitology Research, 2010Identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. The ribosomal second internal transcribed spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) regions are highly conserved in the eukaryotes and are usually used as ...
Bin, Yang, Junlong, Cai, Xunjia, Cheng
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Parasitology Research, 2007
The Anopheles (Anopheles) Hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. Despite their pathological importance, some unidentified internal transcribed spacer 2 (ITS2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. In
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The Anopheles (Anopheles) Hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. Despite their pathological importance, some unidentified internal transcribed spacer 2 (ITS2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. In
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5.8S-28S rRNA interaction and HMM-based ITS2 annotation
Gene, 2009The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal repeat unit is one of the most commonly applied phylogenetic markers. It is a fast evolving locus, which makes it appropriate for studies at low taxonomic levels, whereas its secondary structure is well conserved, and tree reconstructions are possible at higher taxonomic levels. However,
Alexander, Keller +5 more
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Identification of Anopheles daciae in Germany through ITS2 sequencing
Parasitology Research, 2012Until the middle of the twentieth century, malaria was frequently endemic in parts of Germany; Anopheles maculipennis complex species were considered the primary vectors. Three species of this complex have been identified in Germany: A. maculipennis s.s., Anopheles messeae and Anopheles atroparvus; the last predominantly from the coastal regions of ...
Thomas, Weitzel +2 more
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ITS2 Database IV: Interactive taxon sampling for internal transcribed spacer 2 based phylogenies
Molecular Phylogenetics and Evolution, 2012The first step of any molecular phylogenetic analysis is the selection of the species and sequences to be included, the taxon sampling. Already here different pitfalls exist. Sequences can contain errors, annotations in databases can be inaccurate and even the taxonomic classification of a species can be wrong.
Koetschan, Christian +5 more
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ITS2 ribosomal RNA indicates Schistosoma hippopotami is a distinct species
International Journal for Parasitology, 1995The internal transcribed spacer region of the ribosomal RNA, ITS2, was sequenced from a single specimen of S. hippopotami collected from a pulmonary artery of the hippopotamus, Hippopotamus amphibius in South Africa. The nucleotide sequence was aligned with those of S. mansoni, S. rodhaini, S. haematobium, S. intercalatum, S. curassoni, S. bovis and S.
L, Després +3 more
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Identification of medicinal vines by ITS2 using complementary discrimination methods
Journal of Ethnopharmacology, 2012Medicinal vines listed in Chinese pharmacopoeia possess important medicinal efficacy in traditional Chinese medicines.The ITS2 region, which has several characteristics that make it a valuable DNA barcode, was studied to discriminate the stems of medicinal vines to confirm their identities and ensure their safe application in pharmaceuticals by using ...
Zhihua, Liu +8 more
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ITS2 characterization and Anopheles species identification of the subgenus Cellia
Acta Tropica, 2013In Mizoram, the origin and molecular nature of Anopheles species is poorly understood, despite the region having high malarial incidence and rich biodiversity. A diagnostic PCR assay for distinguishing the Cellia subgenera members of Anopheles species was developed based on the interspecific ITS2 variation.
Rita, Zomuanpuii +4 more
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ITS2 is a double-edged tool for eukaryote evolutionary comparisons
Trends in Genetics, 2003Abstract The internal transcribed spacer (ITS) region of the nuclear rDNA cistrons is one of the more frequently utilized regions for phylogenetic analyses at the genus and species levels. It has proven valuable for phylogenetic reconstruction of species and genus relationships, using comparisons of primary sequence.
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