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Untangling ITS2 genotypes of algal symbionts in zooxanthellate corals
Molecular Ecology Resources, 2020AbstractCollectively called zooxanthellae, photosynthetic dinoflagellates in the family Symbiodiniaceae are typical endosymbionts that unequivocally mediate coral responses to environmental changes. Symbiodiniaceae are genetically diverse, encompassing at least nine phylogenetically distinct genera (clades A–I).
Tuo Shi +7 more
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Quantitative analysis of ITS2 sequences in trichostrongyle parasites
International Journal for Parasitology, 2002Real-time PCR assays were developed to identify and quantify common gastrointestinal nematodes of ruminants. The assays were based on genus-specific primer and probe combinations derived from the second internal transcribed spacer (ITS2) ribosomal DNA transcription unit of Haemonchus contortus, Ostertagia leptospicularis, Trichostrongylus colubriformis
G, von Samson-Himmelstjerna +2 more
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Identification of Apiaceae using ITS, ITS2 and psba-trnH barcodes
Molecular Biology Reports, 2022Apiaceae plants are used as medicinal herbs, pesticides, spices, and vegetables; thus, accurately identifying Apiaceae species is important. The grassland ecosystem of Heilongjiang Province in northern China has huge reserves of wild Apiaceae plants, but few reports have systematically documented their diversity.
Zhehui Jiang +8 more
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Phytogeny of genusGlossina (Diptera:Glossinidae) according to ITS2 sequences
Science in China Series C: Life Sciences, 1999The flies of genusGlossina (Diptera: Glossinidae) are an important vector of African trypanosomiases which cause diseases in humans and animals. The ribosomal DNA Internal Transcribed Spacer-2 (ITS-2) region sequences from differentGlossina species were PCR-amplified and analyzed in order to construct a molecular phylogeny for genusGlossina.
X, Chen, S, Li, C, Li, S, Zhao, A, Serap
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Identification of astigmatid mites using ITS2 and COI regions
Parasitology Research, 2010Identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. The ribosomal second internal transcribed spacer (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) regions are highly conserved in the eukaryotes and are usually used as ...
Bin, Yang, Junlong, Cai, Xunjia, Cheng
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Parasitology Research, 2007
The Anopheles (Anopheles) Hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. Despite their pathological importance, some unidentified internal transcribed spacer 2 (ITS2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. In
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The Anopheles (Anopheles) Hyrcanus group of mosquitoes is very important for human health because some are malarial vector mosquitoes. Despite their pathological importance, some unidentified internal transcribed spacer 2 (ITS2) sequences have been reported from members of this group, and their phylogenetic relationships have been rarely understood. In
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5.8S-28S rRNA interaction and HMM-based ITS2 annotation
Gene, 2009The internal transcribed spacer 2 (ITS2) of the nuclear ribosomal repeat unit is one of the most commonly applied phylogenetic markers. It is a fast evolving locus, which makes it appropriate for studies at low taxonomic levels, whereas its secondary structure is well conserved, and tree reconstructions are possible at higher taxonomic levels. However,
Alexander, Keller +5 more
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Identification of Anopheles daciae in Germany through ITS2 sequencing
Parasitology Research, 2012Until the middle of the twentieth century, malaria was frequently endemic in parts of Germany; Anopheles maculipennis complex species were considered the primary vectors. Three species of this complex have been identified in Germany: A. maculipennis s.s., Anopheles messeae and Anopheles atroparvus; the last predominantly from the coastal regions of ...
Thomas, Weitzel +2 more
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ITS2 Database IV: Interactive taxon sampling for internal transcribed spacer 2 based phylogenies
Molecular Phylogenetics and Evolution, 2012The first step of any molecular phylogenetic analysis is the selection of the species and sequences to be included, the taxon sampling. Already here different pitfalls exist. Sequences can contain errors, annotations in databases can be inaccurate and even the taxonomic classification of a species can be wrong.
Koetschan, Christian +5 more
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ITS2 ribosomal RNA indicates Schistosoma hippopotami is a distinct species
International Journal for Parasitology, 1995The internal transcribed spacer region of the ribosomal RNA, ITS2, was sequenced from a single specimen of S. hippopotami collected from a pulmonary artery of the hippopotamus, Hippopotamus amphibius in South Africa. The nucleotide sequence was aligned with those of S. mansoni, S. rodhaini, S. haematobium, S. intercalatum, S. curassoni, S. bovis and S.
L, Després +3 more
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