Results 21 to 30 of about 58,032 (218)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Experimental Study on the Influence of Jet Dynamic Pressure Based on a New Confining Pressure Control System

Shiyou jixie
To address challenges such as low operating efficiency and poor descaling effect in jet stimulation operation of deep/ultra-deep wells， the influence of downhole environmental pressure （confining pressure） on jet dynamic pressure has been studied at home
Zhou Shijie, Lü Weiping, Liu Jiawei, Zhang Zheng, Zhu Ye, Luo Bin   +5 more
doaj   +3 more sources

Experimental study of shock pressure and erosion characteristics of high‐pressure gas–liquid two‐phase jet: Exploration for improving coalbed methane extraction efficiency

Energy Science & Engineering, 2023
Coalbed methane extraction promotes energy exploitation and alleviates the greenhouse effect. This study aimed to analyze the technical potential of a high‐pressure gas–liquid two‐phase jet in increasing coalbed permeability and promoting coalbed methane
Longyong Shu, Yongpeng Fan, Yunlong Yang, Xin Song, Haijun Guo   +4 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

A Numerical Simulation of the Underwater Supersonic Gas Jet Evolution and Its Induced Noise

Applied Sciences, 2023
To explore the complex flow field and noise characteristics of underwater high-speed gas jets, the mixture multiphase model, large eddy simulation method, and Ffowcs Williams–Hawking (FW–H) acoustic model were used for simulations, and the numerical ...
Wei Yu, Baoshou Wang, Chun Zhang
doaj   +1 more source

Distinct Systemic Sclerosis Phenotypes Related to Ethnicity: An Opportunity to Personalize Care?

Arthritis Care &Research, EarlyView.
Objective The objective is to describe and compare demographic, clinical, and serological characteristics of patients with systemic sclerosis (SSc) according to ethnic background. Methods Participants enrolled in the Canadian Scleroderma Research Group cohort who self‐identified to a single ethnicity group were included.
Danick Goulet, Camille Guertin, Maggie Larché, Isabelle Ferdinand, Marie Hudson, May Y. Choi, Mohammed Osman, Janet Pope, Carter Thorne, Murray Baron, Sasha Bernatsky, Sabrina Hoa   +11 more
wiley   +1 more source

Numerical analysis on the flow field structure and deflection characteristics of water jets under nozzle moving conditions

Engineering Applications of Computational Fluid Mechanics, 2020
High-pressure water jets under nozzle moving conditions are widely utilized for rock breakage in geotechnical engineering. The nozzle motion state significantly influences the jet flow field and flow characteristics, thus changing its rock-breaking ...
Songqiang Xiao, Zhaolong Ge, Qingyang Ren, Jialiang Liu, Haiyang Wang   +4 more
doaj   +1 more source

Thermomechanical Fatigue and Creep–Fatigue Interaction of Inconel 718 Additively Manufactured by Laser Beam Powder Bed Fusion

Advanced Engineering Materials, EarlyView.
Thermomechanical fatigue tests of laser beam powder bed fusion (PBF‐LB) Inconel 718 show that the additively manufactured material reaches almost the lifetimes of conventionally‐rolled material under no‐dwell conditions. Introducing dwell times at the maximum temperature markedly reduces the lifetimes due to pronounced grain boundary sliding associated
Stefan Guth, Tomáš Babinský, Jan Lars Riedel, Wen Hao Kan, Aijun Huang, Martin Heilmaier, Ivo Šulák   +6 more
wiley   +1 more source

Flexural Behavior of 3D‐Printed Porous Bouligand‐Structured Polymers: Influence of Pitch Angle and Porosity

Advanced Engineering Materials, EarlyView.
This study examines how helicoidal architectures with different porosities respond to bending. Adjusting layer angle and spacing in 3D‐printed polymers reveals clear tradeoffs between stiffness, strength, and energy absorption. Experiments and simulations highlight designs that distribute stress effectively, offering pathways for optimizing lightweight
Praveenkumar Subhash Patil, Edward D. McCarthy, Parvez Alam   +2 more
wiley   +1 more source

Fabrication Routes for Ionic Conducting Fiber Strain Sensors

Advanced Engineering Materials, EarlyView.
Ionic conducting fiber strain sensors (ICFSs) offer compliant, textile‐integrable sensing. Thus far, the commercialization of ICFSs has been constrained by fiber fabrication routes. This review provides a fabrication‐centric analysis of ICFSs correlating processing strategies with material properties and scalability.
Leo John Kershaw, Zhanhong Yang, Anne Ladegaard Skov, Magdalena Skowyra   +3 more
wiley   +1 more source