Results 1 to 10 of about 50 (50)
Homooligomeric and Heterooligomeric Associations between K+-Cl– Cotransporter Isoforms and between K+-Cl– and Na+-K+-Cl– Cotransporters [PDF]
Journal of Biological Chemistry, 2007 Little is known regarding the quaternary structure of cation-Cl- cotransporters (CCCs) except that the Na+-dependent CCCs can exist as homooligomeric units. Given that each of the CCCs exhibits unique functional properties and that several of these carriers coexist in various cell types, it would be of interest to determine whether the four K+-Cl ...
Charles F. Simard +6 more
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Functional interaction of the K-Cl cotransporter (KCC1) with the Na-K-Cl cotransporter in HEK-293 cells [PDF]
American Journal of Physiology-Cell Physiology, 1999 We have studied the regulation of the K-Cl cotransporter KCC1 and its functional interaction with the Na-K-Cl cotransporter. K-Cl cotransporter activity was substantially activated in HEK-293 cells overexpressing KCC1 (KCC1-HEK) by hypotonic cell swelling, 50 mM external K, and pretreatment with N-ethylmaleimide (NEM). Bumetanide inhibited 86Rb efflux
Christopher M. Gillen, Bliss Forbush
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Characterization of Glial Cell K-Cl Cotransport [PDF]
Cellular Physiology and Biochemistry, 2007 The molecular mechanism of K-Cl cotransport (KCC) consists of at least 4 isoforms, KCC 1, 2, 3, and 4 which, in multiple combinations, exist in most cells, including erythrocytes and neuronal cells.We utilized reverse-transcriptase-polymerase chain reaction (RT-PCR) and ion flux studies to characterize KCC activity in an immortalized in vitro cell ...
Gagnon, Kenneth B. E. +3 more
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Kidney International, 1989 The KCl cotransporter in the basolateral membrane of renal tubules may play a central role in the transcellular transport of NaCl. Because this transporter is electrically neutral, and also functions in parallel to the electrogenic Na,K-ATPase, there is an imbalance in charge which must be expressed as a cationic current across the basolateral membrane.
David G. Warnock, Jill Eveloff
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Comparison of Na-K-Cl Cotransporters [PDF]
Journal of Biological Chemistry, 1998 The Na-K-Cl cotransporter (NKCC) mediates the coupled movement of ions into most animal cells, playing important roles in maintenance of cell volume and in epithelial Cl transport. Two forms of NKCC have been described: NKCC1, the "housekeeping" isoform that is also responsible for Cl accumulation in secretory epithelial cells, and NKCC2, which ...
Bliss Forbush +3 more
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The electroneutral Na+-(K+)-Cl− cotransport family
Kidney International, 1996 Recently the molecular identification of the major electroneutral sodium-potassium-chloride entry mechanisms present on apical membranes of distal nephron segments of the mammalian kidney, on basolateral membranes of many non-renal epithelial cells and on certain non-epithelial tissues has been achieved. These transporters represent a major pathway for
Gerardo Gamba +2 more
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Regulation of K-Cl Cotransport: from Function to Genes [PDF]
Journal of Membrane Biology, 2004 This review intends to summarize the vast literature on K-Cl cotransport (COT) regulation from a functional and genetic viewpoint. Special attention has been given to the signaling pathways involved in the transporter's regulation found in several tissues and cell types, and more specifically, in vascular smooth muscle cells (VSMCs).
Norma C. Adragna +2 more
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Evidence for K‐Cl cotransporter heterodimerization
The FASEB Journal, 2012 In humans, mutations in the gene SLC12A6 which encodes for KCC3 result in an autosomal recessive disease, known as ACCPN (Agenesis of the Corpus Callosum associated with Peripheral Neuropathy). In the present work we focused our efforts in understanding the lethality demonstrated by the homozygosity of the Glu289Gly (E289G) mutation in the mouse K‐Cl ...
Eric Delpire +2 more
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K-Cl Cotransport: Properties and Molecular Mechanism
Cellular Physiology and Biochemistry, 2000 K-Cl cotransport (COT), defined first in red blood cells as the Cl-dependent, ouabain-insensitive bidirectional K transport, encoded by at least four KCC (kalium-chloride-cotransport) genes, is now recognized as a functional and structural reality in all cell membranes. As functional system, K-Cl COT is necessary for volume and ionic homeostasis. Since
Peter K. Lauf, Norma C. Adragna
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Functional Comparison of the K+-Cl−Cotransporters KCC1 and KCC4 [PDF]
Journal of Biological Chemistry, 2000 The K(+)-Cl(-) cotransporters (KCCs) are members of the cation-chloride cotransporter gene family and fall into two phylogenetic subgroups: KCC2 paired with KCC4 and KCC1 paired with KCC3. We report a functional comparison in Xenopus oocytes of KCC1 and KCC4, widely expressed representatives of these two subgroups.
Norma Vázquez +4 more
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