Results 171 to 180 of about 8,135 (198)

Recombinant kinetoplast DNA (kDNA) probe for identifying Leishmania tropica

Acta Tropica, 1992
The paper reports on the construction of a kDNA library with DNA isolated from the WHO reference strain of Leishmania tropica IC-305 and subsequent identification and propagation of recombinant plasmids containing L. tropica kDNA sequences. It also shows that the cloned kDNA sequences can be used as genetic markers in restriction endonuclease, Southern
N N, Massamba, M J, Mutinga
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An analysis of trypanosomatids kDNA minicircle by absolute dinucleotide frequency

Parasitology International, 2013
Trypanosomatid mitochondrial DNA (kDNA) possesses thousands of copies of small circular molecules called minicircles. Due to a high level of nucleotide polymorphism among copies, sequence alignment for species or strain characterization is not appropriate.
Luiza, de Oliveira Ramos Pereira, Adeilton, Brandão   +1 more
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First detection of Leishmania kDNA in canine cerumen samples by qPCR

Veterinary Parasitology, 2016
Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be ...
Silvia, Belinchón-Lorenzo, Juan Carlos, Parejo, Virginia, Iniesta, Javier, Fernández-Cotrina, Rubén, Muñoz-Madrid, Isabel, Monroy, Victoria, Baz, Adela, Gómez-Luque, Francisco Javier, Serrano-Aguilera, José Luis, Barneto, Luis Carlos, Gómez-Nieto   +10 more
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kDNA markers define two major Trypanosoma rangeli lineages in Latin-America

Acta Tropica, 2002
Trypanosoma rangeli is a hemoflagellate parasite of man, domestic and wild animals in Central and South America. The genus Rhodnius is particularly susceptible to infection by T. rangeli and transmission by salivary inoculation has been demonstrated in 12 of 14 nominal species of naturally and experimentally infected insects.
Gustavo A, Vallejo, Felipe, Guhl, Julio C, Carranza, Leyder E, Lozano, Jorge L, Sánchez, José C, Jaramillo, Diego, Gualtero, Nadia, Castañeda, Julio C, Silva, Mário, Steindel   +9 more
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Molecular, cytological, and immunocytochemical study and kDNA sequencing of laryngeal Leishmania infantum infection

Parasitology Research, 2012
Mucosal leishmaniasis is a well-known clinical manifestation of infections mainly caused by New World Leishmania species, especially Leishmania braziliensis (Viannia) in Central and South America. It is extremely uncommon in the world, even in the endemic areas such as Fars Province, Southern Iran.
Ahmad, Oryan, Sadegh, Shirian, Mohammad Reza, Tabandeh, Gholam Reza, Hatam, Mohsen, Kalantari, Yahya, Daneshbod   +5 more
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BIOTINYLATED kDNA FROM Leishmania braziliensis

1989
The lesions caused by the Leishmania braziliensis complex begin as a cutaneous lesion. In some cases, as those produced by L. braziliensis braziliensis. the lesion very often progresses towards destruction of mucouse tissues. In the Amazonian jungle, an area endemic for mucocutaneous Leishmaniasis it is common that people, specially new settlers ...
Martin Lopez, Ysabel Montoya, Alejandro Llanos Cuentas, Jorge Arevalo   +3 more
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Transovarial passage of Leishmania infantum kDNA in artificially infected Rhipicephalus sanguineus

Experimental Parasitology, 2010
Phlebotomine sand flies are the only proven biological vectors of Leishmania parasites. However, Rhipicephalus sanguineus ticks have long been suspected to transmit Leishmania infantum in studies carried out in laboratory and natural conditions. In the present study, 5 microl of L.
Filipe, Dantas-Torres, Thiago F, Martins, Milena, de Paiva-Cavalcanti, Luciana A, Figueredo, Bruna S, Lima, Sinval P, Brandão-Filho   +5 more
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Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis

Diagnostic Microbiology and Infectious Disease, 2005
The utility of 2 polymerase chain reaction (PCR)-based assays amplifying genus or Viannia subgenus Leishmania minicircle kDNA for the diagnostics of ML was assessed. The Viannia subgenus product was yielded after PCR from isolates of L. (Viannia) braziliensis, L. (Viannia) colombiensis, and L.
Jolande, Disch, Mariana Junqueira, Pedras, Marcela, Orsini, Claude, Pirmez, Maria Cláudia, de Oliveira, Marcelo, Castro, Ana, Rabello   +6 more
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Identification and Differentiation of Iranian Leishmania Species by PCR Amplification of kDNA

Scandinavian Journal of Infectious Diseases, 2001
We describe the specific identification of Leishmania species in Iran using PCR DNA amplification of kDNA. For this purpose, we designed a pair of primers--upstream 5' TCGCAGAACGCCCCTACC 3' and downstream 5'-AGGGGTTGGTGTAAAATAGGC 3'--specific for conserved sequences of kDNA of Leishmania. Using this primer, we identified 3 different amplified fragments
F, Mahboudi, M, Abolhassan, M, Yaran, H, Mobtaker, M, Azizi   +4 more
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Discrimination of Leishmania braziliensis Variants by kDNA Signatures Produced by LSSP-PCR

Journal of Parasitology, 2007
The conventional methods for identification and typing of Leishmania species depend on previous culture isolation of the parasites. Not infrequently, culture is unsuccessful and may result in misrepresentation of the heterogeneity of the original isolate. Thus, more reliable and precise identification of genotypes of Leishmania spp.
Germano A, Ferreira, Fábia C S, Soares, Slanney A, Vasconcellos, Eduardo H G, Rodrigues, Roberto P, Werkhäuser, Maria Edileuza F, de Brito, Frederico G C, Abath   +6 more
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