Results 181 to 190 of about 8,135 (198)

Detection of Leishmania kDNA in human serum samples for the diagnosis of visceral leishmaniasis

Transactions of the Royal Society of Tropical Medicine and Hygiene, 2009
The performance of PCR to detect Leishmania kDNA in serum for the diagnosis of visceral leishmaniasis (VL) was assessed in serum samples from 65 patients with VL, 17 non-infected individuals and 17 patients with other febrile hepatosplenic diseases. Serum PCR showed a sensitivity of 85%, specificity of 100% and efficiency of 90%. The sensitivity values
Tália Santana Machado, de Assis, Rachel Basques, Caligiorne, Gustavo Adolfo Sierra, Romero, Ana, Rabello   +3 more
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The kinetoplast DNA of Trypanosoma gambiense: Comparison with the kDNA of Trypanosoma equiperdum

Biochimie, 1981
The molecular components of the kinetoplast DNA (kDNA) network of Trypanosoma gambiense have been studied and compared with those of the very closely related species T. equiperdum, previously studied in detail. The kDNA of T. gambiense contains about 80 maxicircles of 20 kilobase pairs and 4000 minicircles of 1 kilobase pairs.
G, Riou, M, Barrois
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Gold nanoparticles-based biosensing of Leishmania major kDNA genome: Visual and spectrophotometric detections

Sensors and Actuators B: Chemical, 2016
Abstract Precise and sensitive identification methods of Leishmania are required for efficient treatment of leishmaniasis. Detection of Leishmania species approves the efficiency of the applied therapy. Current diagnosis methods, such as culture and microscopy methods, are time-consuming and have limited sensitivity, and are laborious when ...
N. Sattarahmady, A. Movahedpour, H. Heli, G.R. Hatam   +3 more
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Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal

Parasitology International, 2006
Around the Mediterranean basin Leishmania infantum is an important parasite causing canine leishmaniasis and visceral and cutaneous clinical forms in both immunocompetent and immunocompromised humans. Efficient monitoring and evaluation of epidemiology with discriminatory molecular markers are required.
Sofia, Cortes, Isabel, Mauricio, Ana, Almeida, José Manuel, Cristovão, Francine, Pratlong, Jean Pierre, Dedet, Lenea, Campino   +6 more
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Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes

Acta Tropica, 1999
Here we define a new approach for the detection and characterisation of Leishmania complexes by polymerase chain reaction (PCR) and specific hybridisation. The first step consists of PCR amplification of kDNA minicircles using general kinetoplastid primers, which generate a polymorphic multi-banding pattern for all Leishmania species and other ...
/Brenière, Simone Frédérique, Telleria, J., /Bosseno, Marie-France, Buitrago, R., /Bastrenta, Brigitte, /Cuny, Gérard, /Banuls, Anne-Laure, Brewster, S., Barker, D.C.   +8 more
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The heterogeneity of choanomastigote-shaped trypanosomatids as analyzed by their kDNA minicircle size: taxonomic implications

Parasitology Research, 2000
The kDNA minicircle size was analyzed in 15 species of choanomastigote-shaped trypanosomatids and four main groups of species were identified: (1) "Crithidia" deanei, "C." desouzai and "Herpetomonas" roitmani, which presented the largest molecules (> or = 3,800 bp), (2) "C." oncopelti with minicircles of at least four different sizes within 1,300-2,650
A A, Brandão, A, Miranda, W M, Degrave, M A, Sousa   +3 more
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Leishmania infantum species-specific kDNA probes: isolation and evaluation.

Archives de l'Institut Pasteur de Tunis, 2003
The study refers to the isolation of specific DNA probes to the parasite species Leishmania (L) infantum according to different strategies using recombinant minicircles isolated from L. infantum kinetoplast DNAs. A first probe was identified following a classical procedure. One mini-circle selected for strong reactivity to L.
A, Ben Hamouda, I, Guizani, S, Jedidi, K, Dellagi, R, Ben Ismaïl   +4 more
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Possible integration of Trypanosoma cruzi kDNA minicircles into the host cell genome by infection

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1994
Infection with Trypanosoma cruzi is known to induce the division of peritoneal macrophages in BALB/c mice. We have demonstrated, by cytogenetic analysis, that accessory DNA elements are associated with the metaphase macrophage chromosomes of such infected macrophages. The identification of these accessory DNA elements with T.
A R, Teixeira, E R, Argañaraz, L H, Freitas, Z G, Lacava, J M, Santana, H, Luna   +5 more
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kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients

Transactions of the Royal Society of Tropical Medicine and Hygiene, 2008
Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T. cruzi kDNA minicircle gene signatures using the low-stringency single-specific-primer PCR technique in both peripheral blood and oesophageal mucosa from chronic chagasic ...
Manoel-Caetano, Fernanda da Silva, Aparecida Carareto, Claudia Marcia, Borim, Aldenis Albaneze, Miyazaki, Kenji, Silva, Ana Elizabete   +4 more
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Intracellular Localization of a Trypanosoma cruzi kDNA Minicircle Transcript Using RNA: RNA In Situ Hybridization

Journal of Eukaryotic Microbiology, 1994
ABSTRACT. Using RNA: RNA in situ hybridization, the intracellular location of a transcript encoded by and spanning the entire length of a Trypanosoma cruzi kinetoplast DNA minicircle was determined. In axenically cultured T. cruzi epimastigotes, the hybridization signal was restricted to the kinetoplast, which was situated in the perinuclear region of
R, Thertulien, P J, Simpson-Haidaris, C G, Haidaris   +2 more
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