Results 51 to 60 of about 8,135 (198)
Revista do Instituto de Medicina Tropical de São Paulo, 2016 In visceral leishmaniasis, the detection of the agent is of paramount importance to identify reservoirs of infection. Here, we evaluated the diagnostic attributes of PCRs based on primers directed to cytochrome-B (cytB), cytochrome-oxidase-subunit II ...
Estela Gallucci LOPES +6 more
doaj +1 more source
Molecular and Functional Characteristics of DNA Polymerase Beta-Like Enzymes From Trypanosomatids
Frontiers in Cellular and Infection Microbiology, 2021 Trypanosomatids are a group of primitive unicellular eukaryotes that can cause diseases in plants, insects, animals, and humans. Kinetoplast genome integrity is key to trypanosomatid cell survival and viability.
Edio Maldonado +3 more
doaj +1 more source
LSSP-PCR of Trypanosoma cruzi: how the single primer sequence affects the kDNA signature [PDF]
BMC Research Notes, 2013 Abstract Background Low-stringency single specific primer PCR (LSSP-PCR) is a highly sensitive and discriminating technique that has been extensively used to genetically characterize Trypanosoma cruzi populations in the presence of large amounts of host DNA. To ensure high sensitivity, in most T.
Segatto, Marcela +5 more
openaire +2 more sources
Novel CRISPR-based detection of Leishmania species
Frontiers in Microbiology, 2022 Tegumentary leishmaniasis, a disease caused by protozoan parasites of the genus Leishmania, is a major public health problem in many regions of Latin America.
Eva Dueñas +11 more
doaj +1 more source
Programmable DNA looping using engineered bivalent dCas9 complexes [PDF]
, 2017 DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA
Dodd, I., Hao, N., Shearwin, K.
core +2 more sources
Iranian Journal of Microbiology, 2021 Background and Objectives: The three old world Leishmania species i.e., L. major, L. tropica, and L. infantum are considered as potential etiological agents of the various clinical forms of leishmaniasis in Iran. Different species co-exist in some areas.
Shima Fayaz +4 more
doaj
A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis
Asian Pacific Journal of Tropical Medicine, 2020 Objective: To determine an algorithm for molecular diagnosis of visceral leishmaniasis (VL) by kinetoplast DNA (kDNA) (RV1/ RV2) and internal transcriber spacer (ITS1) (LITSR/L5.8S) polymerase chain reaction (PCR), complemented by ITS1 PCR restriction ...
Natalia Souza de Godoy +5 more
doaj +1 more source
Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari : Ixodidae) collected from naturally infected dogs [PDF]
, 2015 Background: The main transmission route of Leishmania infantum is through the bites of sand flies. However, alternative mechanisms are being investigated, such as through the bites of ticks, which could have epidemiological relevance.
Araújo, Nadjar Nitz Silva Lociks de +9 more
core +2 more sources
Medical and Veterinary Entomology, EarlyView.This study was to describe the composition of sand fly fauna, detect potential vectors of Leishmania spp. in the Tinguá district, an ATL endemic, Rio de Janeiro, Brazil. Seven species of vectorial importance in the epidemiological cycle of leishmaniasis was observed: Nyssomyia intermedia, Migonemyia migonei, Pintomyia fischeri, Psychodopygus hirsutus ...
Antônio L. F. Santana +9 more
wiley +1 more source
Linajes de Trypanosoma cruzi en pacientes con enfermedad de Chagas y coinfección por VIH [PDF]
, 2009 Introducción. Las poblaciones naturales de T. cruzi han sido clasificadas en seis linajes filogenéticos o unidades de tipificación discreta: T. cruzi I, IIa, IIb, IIc, IId y IIe, que pueden jugar un rol en el tropismo tisular y patogénesis de la ...
Altcheh, Jaime Marcelo +11 more
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