Results 121 to 130 of about 906,870 (314)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Experimental Simulation of Proppant Migration for Slick Water With Variable Viscosity During Fracturing

Geofluids
The migration law of proppants in slick water during fracturing is of great significance for field fracturing. A large-scale visualized experimental device was utilized to test sand patterns for varying injection parameter combinations, and sensitivity ...
Guiquan Wang, Jingyuan Zhao, Yuxue Sun, Anqi Shen   +3 more
doaj   +1 more source

Threshold Key-Recovery Systems for RSA

, 1997
Although threshold key-recovery systems for the discrete log based cryptosystems such as the ElGamal scheme have been proposed by Feldman and Pedersen [6, 11, 12], no (practical) threshold key-recovery system for the factoring based cryptosystems such as
Tatsuaki Okamoto
core  

Key recovery in a business environment [PDF]

, 2001
This thesis looks at the use of key recovery primarily from the perspective of business needs, as opposed to the needs of governments or regulatory bodies.
Rantos, Konstantinos
core  

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Enhancing oil recovery of ultimate water-cut reservoirs with a novel methane-producing bacterial strain

Shiyou shiyan dizhi
Most of the old oil fields in eastern China are now in the ultra-high water-cut development stage characte-rized by a rapid increase in water-cut, low oil recovery rate and inefficient water flooding.
Mingshan DING, Junzhang LIN, Yun FENG, Nan SUN, Guan WANG, Yan BA, Weidong WANG   +6 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Water Shutoff with Polymer Gels in a High-Temperature Gas Reservoir in China: A Success Story

Energies
Gel treatments have been widely applied to control water production in oil and gas reservoirs. However, for water shutoff in dense gas reservoirs, most gel-based treatments focus on individual wells rather than the entire reservoir, exhibiting limited ...
Tao Song, Hongjun Wu, Pingde Liu, Junyi Wu, Chunlei Wang, Hualing Zhang, Song Zhang, Mantian Li, Junlei Wang, Bin Ding, Weidong Liu, Jianyun Peng, Yingting Zhu, Falin Wei   +13 more
doaj   +1 more source