Results 41 to 50 of about 114,382 (262)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Isomerization of an enzyme-coenzyme complex in yeast alcohol dehydrogenase-catalysed reactions [PDF]

Journal of the Serbian Chemical Society, 2003
In this work, all the rate constants in the kinetic mechanism of the yeast alcohol dehydrogenase-catalyzed oxidation of ethanol by NAD+, at pH 7.0, 25 ºC, have been estimated. The determination of the individual rate constants was achieved by fitting the
Leskovac Vladimir M., Trivić Svetlana S., Peričin Draginja M.   +2 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

The reactivity of α,β-unsaturated carboxylic acids. Part XVI. The kinetics of the reaction of cycloalkenecarboxylic and cycloalkenylacetic acids with diazodiphenylmethane in various alcohols [PDF]

Journal of the Serbian Chemical Society, 2002
The rate constants for the reaction of diazodiphenylmethane with 1-cyclopentenecarboxylic, 1-cycloheptenecarboxylic, cyclopent-1-enylacetic and cyclohept-1-enylacetic acids were determined in eight alcohols at 30 ºC using the appropriate UV-spectroscopic
Ušćumlić Gordana S., Nikolić Jasmina B., Krstić Vera V.   +2 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Kinetic behavior of nitrification in the post-treatment of poultry wastewater in a sequential batch reactor Comportamento cinético da nitrificação em um reator em bateladas sequenciais usado no pós-tratamento de efluente avícola

Engenharia Agrícola, 2011
A sequential batch reactor with suspended biomass and useful volume of 5 L was used in the removal of nutrients and organic matter in workbench scale under optimal conditions obtained by central composite rotational design (CCRD), with cycle time (CT) of
Juliana B. R. Mees, Simone D. Gomes, Márcio A. Vilas Boas, Benedito M. Gomes, Fernando H. Passig   +4 more
doaj   +1 more source

Exploring solution ICAR ATRP of methyl acrylate with AIBN as azo initiator: a kinetic modeling and simulation study

Results in Materials, 2022
Supplementary techniques to atom transfer radical polymerization (ATRP) have been studied to reduce the concentration of catalytic complexes used, aiming at a less costly and more environmentally friendly process for synthesizing polymeric materials ...
Guilherme C.L. Faria, Emerson P. Lyra
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Quantum kinetic equations approach to the description of chemical reactions between adparticles at a metallic surface

Condensed Matter Physics, 2006
Adparticle dynamics at a metallic surface is studied by means of quantum kinetic equations. Kinetic equations of the reaction-diffusion type are written down for nonequilibrium one-particle distribution functions of the adsorbate up to the second order ...
V.V.Ignatyuk, M.V.Tokarchuk, P.P.Kostrobij   +2 more
doaj   +1 more source

A Kinetic Approach to the Friction Constant of a Brownian Particle [PDF]

Progress of Theoretical Physics, 1977
The density expansion of the friction constant of a Brownian particle in a gas is inves­ tigated with the use of the resolvent operator formalism of the time correlation function for the transport coefficient. To lowest order in density, the results are identical with those derived from the Boltzmann equation. A divergence appears in the first (second)
openaire   +2 more sources