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Methods for Determining Glycosyltransferase Kinetics
2017Glycosyltransferases are a class of biosynthetic enzymes that transfer individual activated monosaccharide units to specific acceptors. Colorimetric assays using the detection of released products such as para-nitrophenol and coupled assays for inorganic phosphate detection allow for convenient and quantifiable kinetic characterization.
Maria, Ngo, Michael D L, Suits
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Kinetic CE: Foundation for homogeneous kinetic affinity methods
ELECTROPHORESIS, 2007AbstractKinetic capillary electrophoresis (KCE) is defined as capillary electrophoresis of species that interact during electrophoresis. KCE can serve as a conceptual platform for development of homogeneous kinetic affinity methods for affinity measurements (measurements of binding parameters and quantitative measurements) and affinity purification ...
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An alternative method for manipulator kinetic analysis-the D'Alembert method
Proceedings. 1988 IEEE International Conference on Robotics and Automation, 2003The method presented can be applied to both closed-chain and open-chain manipulators. It is proved to be more computationally efficient that the conventional Newton-Euler method in inverse manipulator kinetics analysis. The improvement in computational efficiency is due to two factors: First, the method takes advantage of the special geometry of the ...
Shin-Min Song, Yueh-Jaw Lin
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Kinetic method for microdetermination of the insecticide phosalone
Talanta, 1981The alkaline hydrolysis of the insecticide S-([6-chloro-2-oxo-3(2H)-benzoxazolyl]methyl)-O, O-diethylphosphorodithioate (phosalone) results in O,O-diethylphosphorothioate and 6-chloro-3-mercaptomethylbenzoxazolone. Phosalone and its hydrolysis products act as activators in the copper(II) catalysis oxidation of hydroquinone by hydrogen peroxide in ...
G, Petrov, D, Bachvarova
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Numerical Methods for Modeling Enzyme Kinetics
2021Differential equations are used to describe time-dependent changes in enzyme kinetics and pharmacokinetics. Analytical and numerical methods can be used to solve differential equations. This chapter describes the use of numerical methods in solving differential equations and its applications in characterizing the complexities observed in enzyme ...
Jaydeep, Yadav +2 more
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Convenient method for studying enzyme kinetics
Biotechnology and Bioengineering, 1979AbstractA convenient method for enzyme kinetic studies is introduced. The method includes identification of reaction mechanism and estimation of the associated kinetic constants with a minimum number of experiments. The application of the method is illustrated by using literature data. Factors limiting the application of this method are also discussed.
T A, Hsu, G T, Tsao
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2012
As all classic methods for kinetic analysis of data from thermal analysis experiments, the nonparametric kinetics method, NPK, assumes that the general expression for the reaction rate of a simple reaction is $$ {\dot{\alpha } = g(\alpha ) \cdot f(T)} $$ (16.1) where g(α) symbolizes the kinetic model of the process and f(T) accounts for the ...
Julià Sempere +3 more
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As all classic methods for kinetic analysis of data from thermal analysis experiments, the nonparametric kinetics method, NPK, assumes that the general expression for the reaction rate of a simple reaction is $$ {\dot{\alpha } = g(\alpha ) \cdot f(T)} $$ (16.1) where g(α) symbolizes the kinetic model of the process and f(T) accounts for the ...
Julià Sempere +3 more
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Ionization Energy Determination by the Kinetic Method
Analytical Chemistry, 1996Ionization energies of organic compounds can be determined by the kinetic method by dissociation of radical cations of van der Waals complexes. The ionized dimeric complexes of benzene and substituted benzenes, generated in the ion source of a multiquadrupole instrument by gentle charge exchange chemical ionization using carbon disulfide as reagent gas,
P S, Wong, S, Ma, R G, Cooks
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Schematic methods for probabilistic enzyme kinetics
Journal of Theoretical Biology, 1992The theory of steady-state enzyme processes which avoids using the mass action law of chemical kinetics and consistently describes catalytic mechanisms by probabilistic concepts has recently been proposed (Mazur, 1991, J. theor. Biol. 148, 229-242). To facilitate the analysis of complex reaction graphs by this theory the possibility of constructing ...
A K, Mazur, A V, Kuchinski
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Journal of Molecular Catalysis, 1983
Abstract Transient kinetic methods allow for assessment of the reactivity and the abundancy of catalyst-bound intermediates. These methods are therefore instrumental in placing the comparison between heterogeneous and homogeneous catalytic pathways on a quantitative basis. The following is a brief analysis of transient kinetic data and of the factors
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Abstract Transient kinetic methods allow for assessment of the reactivity and the abundancy of catalyst-bound intermediates. These methods are therefore instrumental in placing the comparison between heterogeneous and homogeneous catalytic pathways on a quantitative basis. The following is a brief analysis of transient kinetic data and of the factors
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