Results 81 to 90 of about 402,340 (304)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

The Effect of Temperature on the Kinetic Parameters of TeF6 Production [PDF]

open access: yesJournal of Nuclear Research and Applications
This study examined the kinetics of the reaction between tellurium oxide (TeO2) and fluorine gas (F2) to produce tellurium hexafluoride gas (TeF6). This investigation was conducted between 150 and 250 °C and between 1 and 2 bar of pressure.
P. Zaheri, A. Charkhi, I. Dehghan
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Transesterification Kinetics of Waste Vegetable Oil in Supercritical Alcohols

open access: yesEnergies, 2014
The kinetic analysis method using the non-isothermal technique was proposed to determine the kinetic parameters for the transesterification reaction of waste vegetable oil (WVO) in supercritical alcohols.
Nawaraj Sanjel, Jae Hoi Gu, Sea Cheon Oh
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

A lumped kinetic model for heavy fuel oil combustion [PDF]

open access: yes, 2015
The study proposes a new procedure based on the Coats-Redfern method to develop kinetic model for predicting the combustion characteristics of heavy fuel oils.
Han, Yunqing   +3 more
core  

Methods of nonlinear kinetics

open access: yes, 2003
Significantly extended version with lattice Boltzmann method and chemical ...
Gorban, A. N., Karlin, I. V.
openaire   +2 more sources

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

open access: yesFEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay   +2 more
wiley   +1 more source

Continuum modelling of granular particle flow with inelastic inter-particle collisions [PDF]

open access: yes, 2003
The kinetic theory of granular flow is a successful model for gas-solid flows. However, inelastic collisions between particles, among other mechanisms, cause agglomeration of particles, which may be the reason why undue sensitivity of the model to any ...
Zhang, Y.H., Reese, J.M.
core   +1 more source

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