Results 121 to 130 of about 2,068 (144)

Extended knobs-into-holes packing in classical and complex coiled-coil assemblies☆

Journal of Structural Biology, 2003
This year marks the 50th anniversary of Crick's seminal paper on the packing of alpha-helices into coiled-coil structures. The central tenet of Crick's work is the interdigitation of side chains, which directs the helix-helix interactions; so called knobs-into-holes packing.
Walshaw, J, Woolfson, DN
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Interaction of transmembrane helices by a knobs-into-holes packing characteristic of soluble coiled coils

Proteins: Structure, Function, and Genetics, 1998
Membrane-embedded protein domains frequently exist as alpha-helical bundles, as exemplified by photosynthetic reaction centers, bacteriorhodopsin, and cytochrome C oxidase. The sidechain packing between their transmembrane helices was investigated by a nearest-neighbor analysis which identified sets of interfacial residues for each analyzed helix-helix
D, Langosch, J, Heringa
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Snap helix with knob and hole: Essential repeats in S. pombe nuclear protein nuc2 +

Cell, 1990
The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like fraction. The nuc2+ protein contains a domain, separated from ten 34 amino acid repeat segments, that is capable of binding AT-rich DNA in vitro.
T, Hirano, N, Kinoshita, K, Morikawa, M, Yanagida   +3 more
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Extraction and Characterisation of Molecular Surface Knobs and Holes for the Study of Complex Formation

1994
Publisher Summary This chapter reviews the extraction and characterization of molecular surface knobs and holes for the study of complex formation. In a study described in the chapter, the contour maps for trypsin–trypsin inhibitor complex were defined.
Anne Badel-Chagnon, Serge Hazout
openaire   +1 more source

Rapid Identification of Disulfide Bonds and Cysteine-Related Variants in an IgG1 Knob-into-Hole Bispecific Antibody Enhanced by Machine Learning

Analytical Chemistry, 2018
Bispecific antibodies are regarded as the next generation of therapeutic modalities as they can simultaneously bind multiple targets, increasing the efficacy of treatments for several diseases and opening up previously unattainable treatment designs. Linking two half antibodies to form the knob-into-hole bispecific antibody requires an additional in ...
Jordan J. Baker, Dana McDaniel, David Cain, Paula Lee Tao, Charlene Li, Yuting Huang, Hongbin Liu, Judith Zhu-Shimoni, Milady Niñonuevo   +8 more
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Structural differences between glycosylated, disulfide-linked heterodimeric Knob-into-Hole Fc fragment and its homodimeric Knob-Knob and Hole-Hole side products.

Protein engineering, design & selection : PEDS, 2017
An increasing number of bispecific therapeutic antibodies are progressing through clinical development. The Knob-into-Hole (KiH) technology uses complementary mutations in the CH3 region of the antibody Fc fragment to achieve heavy chain heterodimerization.
Kuglstatter, A., Stihle, M., Neumann, C., Müller, C., Schaefer, W., Klein, C., Benz, J.   +6 more
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Knobs-into-Holes Packing of α-helices: Coiled Coils and Beyond

Biochemical Society Transactions, 2000
J. Walshaw, D. N. Woolfson
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Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single-capture chromatography

Bioresources and Bioprocessing, 2022
Serene W Chen, Farouq Bin Mahfut
exaly  

assembly of fibrinogen/hyaluronic acid hydrogel via knob-hole interaction for 3D cellular engineering

Bioactive Materials, 2017
Changyou Gao, Lie Ma
exaly  

‘Knobs-into-holes’ provides a rational design strategy for engineering antibody CH3 domains for heavy chain heterodimerization

Immunotechnology, 1996
P. Carter, J.B.B. Ridgway, L.G. Presta
openaire   +1 more source