Results 21 to 30 of about 900,349 (201)

Experience With Performing Rheocarna Therapy via the Single‐Needle Method for Treatment of Chronic Limb‐Threatening Ischemia

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction This study investigated the safety and efficacy of single‐needle Rheocarna therapy for chronic limb‐threatening ischemia (CLTI) with wounds. Methods Six patients with CLTI involving ulcers unresponsive to revascularization underwent single‐needle Rheocarna treatment.
Yasutaka Yamauchi   +9 more
wiley   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

open access: yesFEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

open access: yesFEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura   +2 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

open access: yesFEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa   +2 more
wiley   +1 more source

MEDICIONES SISTEMÁTICAS DE RADIACIÓN UVE - OTOÑO 2011

open access: yesAnales (Asociación Física Argentina), 2013
Durante el año 2010 se realizaron mediciones de prueba con el medidor de Radiación UVE (correspondiente a la radiación Ultra Violeta en la banda UV dosis Eritémica).
M Lavorato   +7 more
doaj  

Advantages of Femtosecond Laser Microdrilling PDMS Membranes over Conventional Methods for Organ-on-a-Chip

open access: yesJournal of Manufacturing and Materials Processing
Organ-on-a-chip (OoC) technology aims to replicate the functions of human organs and tissues. This study evaluates femtosecond laser micromachining (FLM) for producing PDMS membranes with controlled porosity as an alternative approach to conventional ...
Chahinez Berrah   +3 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

MÉTODOS DE ESTUDIO DE CAPA LÍMITE ATMOSFÉRICA (CLA) MEDIANTE UN LIDAR DE RETRODIFUSIÓN

open access: yesAnales (Asociación Física Argentina), 2013
Se estudian distintos métodos para la determinación de la CLA (Capa Limite Atmosférica) mediante un Lidar de Retrodifusión y el rango de validez de los datos.
J. Fochesatto   +5 more
doaj  

Ultrafast sub-nanometer matter-wave temporal Talbot effect

open access: yesNew Journal of Physics, 2021
The coherent manipulation of the electron wavefunction at the atomic spatial and temporal scales is the fundamental breakthrough underlying far-reaching ultrafast phenomena as high-order harmonic radiation and attosecond pulse generation.
Ana García-Cabrera   +2 more
doaj   +1 more source

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