Results 51 to 60 of about 20,611,816 (251)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Pattern Formation Induced by Fuzzy Fractional-Order Model of COVID-19

Axioms, 2022
A novel coronavirus infection system is established for the analytical and computational aspects of this study, using a fuzzy fractional evolution equation (FFEE) stated in Caputo’s sense for order (1,2).
Abeer S. Alnahdi, Ramsha Shafqat, Azmat Ullah Khan Niazi, Mdi Begum Jeelani   +3 more
doaj   +1 more source

Application of the Extended Kalman filter to fuzzy modeling: Algorithms and practical implementation [PDF]

, 2011
Modeling phase is fundamental both in the analysis process of a dynamic system and the design of a control system. If this phase is in-line is even more critical and the only information of the system comes from input/output data.
Jose Manuel Andujar Marquez, Andujar Marquez, Jose Manuel, Aznar Torres, Mariano Jose, Al-Hadithi, Basil Mohammed, Barragán Piña, Antonio Javier, Antonio Javier Barragan Pina, Al-Hadithi, Basil M., Aznar Torres, Mariano J., Jiménez Avello, Agustín, Agustin Jimenez, Basil M Al-Hadithi, Mariano J. Aznar Torres   +11 more
core   +1 more source

Neurologic Manifestations of Long COVID Affect Adult Females More Severely Than Males

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To characterize differences in neurologic manifestations of postacute sequelae of SARS‐CoV‐2 infection (Neuro‐PASC) between females and males. Methods Cross‐sectional study of the first consecutive 261 posthospitalization Neuro‐PASC (PNP) and 2068 nonhospitalized Neuro‐PASC (NNP) patients evaluated at the Neuro‐COVID clinic between ...
Hannah Kopinsky, Melissa Lopez, Janet Miller, Millenia Jimenez, Eric M. Liotta, Igor J. Koralnik   +5 more
wiley   +1 more source

Cracking the Code: Which Ocular Symptoms Predict Dry Eye Signs? Insights From a Large International Sicca Registry

Arthritis Care &Research, EarlyView.
Objective The study aimed to identify symptom‐based predictors of dry eye disease (DED) signs in the Sjögren's International Collaborative Clinical Alliance (SICCA) cohort. Methods We performed a retrospective analysis examining 16 ocular symptoms (most graded 0–4) and artificial tear (AT) use (graded 0–3) as predictors of DED signs (abnormal ocular ...
Pragnya R. Donthineni, Chloe Shields, Rohit Muralidhar, Shreya Bhatt, Alan Baer, Robert Fox, Sara S. McCoy, Anat Galor   +7 more
wiley   +1 more source

Prescribed time fuzzy control for constrained multiagent systems with unknown control direction

工程科学学报
In recent years, multiagent systems (MASs) have completed numerous complex tasks through effective communication and coordination among agents. This has enhanced MAS’s robustness and controllability, leading to their widespread application in practical ...
Fei LI, Chao ZHOU, Lirong FAN, Fang WANG
doaj   +1 more source

Heat Transfer Performance in a Superheater of an Industrial CFBC Using Fuzzy Logic-Based Methods

Entropy, 2019
The heat transfer coefficient in the combustion chamber of industrial circulating flidized bed (CFB) boilers depends on many parameters as it is a result of multifactorial mechanisms proceeding in the furnace.
Jaroslaw Krzywanski
doaj   +1 more source

Empirical Studies on Software Product Maintainability Prediction: A Systematic Mapping and Review [PDF]

e-Informatica Software Engineering Journal, 2019
Background: Software product maintainability prediction (SPMP) is an important task to control software maintenance activity, and many SPMP techniques for improving software maintainability have been proposed.
Sara Elmidaoui, Laila Cheikhi, Ali Idri, Alain Abran   +3 more
doaj   +1 more source