Results 31 to 40 of about 440,327 (194)
High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy
Journal of Lipid Research, 2018 In vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency to float. Assessing adipocyte differentiation using current microscopy techniques involves cell staining and washing, while using flow cytometry involves cell
Vasco Campos +4 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Label-Free Optical Resonator-Based Biosensors
Sensors, 2020 The demand for biosensor technology has grown drastically over the last few decades, mainly in disease diagnosis, drug development, and environmental health and safety.
Donggee Rho +2 more
doaj +1 more source
FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
Cancer Informatics, 2008 Recently, several research groups have published methods for the determination of proteomic expression profiling by mass spectrometry without the use of exogenously added stable isotopes or stable isotope dilution theory.
Soyoung Ryu +5 more
doaj +2 more sources
Label free, quantitative single-cell fate tracking of time-lapse movies
MethodsX, 2019 Historically, the ability to perform multi-day time-lapse imaging of adherent cells required expensive and specialized microscopy equipment. As byproduct of this cost, many labs would synchronize cells using inhibitors such as hydroxyurea and thymidine ...
C. Elizabeth Caldon, Andrew Burgess
doaj +1 more source
3D structured biochip for label free determinations at the point-of-need
Micro and Nano Engineering, 2022 A novel optical label-free biosensing approach is introduced aiming to remove the current limitations of optical biosensors in their application of on-site food analysis.
G. Zisis +5 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Analytical Science Advances, 2020 Surface‐enhanced Raman spectroscopic (SERS) approaches are emerging for bacteria analysis whereby bacteria cells can be measured based on their biochemical composition (label‐free) or with the aid of a chemical label to enhance the SERS signal. Combining
Michael E. Hickey, Siyue Gao, Lili He
doaj +1 more source
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source