Results 41 to 50 of about 440,327 (194)
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Information‐Distilled Generative Label‐Free Morphological Profiling Encodes Cellular Heterogeneity
Advanced ScienceImage‐based cytometry faces challenges due to technical variations arising from different experimental batches and conditions, such as differences in instrument configurations or image acquisition protocols, impeding genuine biological interpretation of ...
Michelle C. K. Lo +7 more
doaj +1 more source
Beyond fluorescence: A critical look at AI-powered brightfield analysis for T-cell killing assays
SLAS DiscoveryThis comment evaluates dela Cruz-Chuh et al.’s AI-based label-free workflow for analyzing T-cell mediated tumor killing via brightfield imaging.
Xiaoman Liu, Zihan Gao, Jiachun Xu
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Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Advanced Sensor ResearchSurface enhanced Raman spectroscopy (SERS) is becoming an attractive analytical technique for the next generation of breath diagnostics. However, current SERS substrates present challenges related to fabrication cost, complexity, signal uniformity, and ...
Marios Constantinou +6 more
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6. Label-free selective plane illumination microscopy of tissue samples
Journal of the Saudi Heart Association, 2017 Basic Science Research. Presentation type: Poster Presentation. Introduction: There is an increasing demand for non-invasive, label-free, three-dimensional imaging techniques for characterisation of optically turbid samples such as tissue engineered ...
Muteb Alharbi +3 more
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Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Small ScienceThe evolution of label‐free electrochemical biosensors has revolutionized the field of analytical detection by enabling rapid, direct, and sensitive detection of a wide range of analytes.
Nandhinee Radha Shanmugam +4 more
doaj +1 more source