Results 101 to 110 of about 4,376,632 (304)
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Label-Free Bioaerosol Sensing Using Mobile Microscopy and Deep Learning
, 2018 Conventional bioaerosol sensing requires the sampled aerosols in the field to be transferred to a laboratory for manual inspection, which can be rather costly and slow, also requiring a professional for labeling and microscopic examination of the samples.
Yibo Zhang (288948) +23 more
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Tools for Label-free Peptide Quantification
Molecular & Cellular Proteomics, 2013 The increasing scale and complexity of quantitative proteomics studies complicate subsequent analysis of the acquired data. Untargeted label-free quantification, based either on feature intensities or on spectral counting, is a method that scales particularly well with respect to the number of samples.
S. Nahnsen +3 more
openaire +3 more sources
Translophagy—A potential link between autophagy impairment and translational errors
FEBS Letters, EarlyView.Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk +11 more
wiley +1 more source
Simple quantitation and spatial characterization of label free cellular images
HeliyonLabel-free imaging is routinely used during cell culture because of its minimal interference with intracellular biology and capability of observing cells over time.
Vincent C.J. de Boer, Xiang Zhang
doaj +1 more source
, 2010 Label-free chemical/biochemical sensing device based on an integrated microfluidic channel within a waveguide ...
MI Mohammed (14541086) +6 more
core
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Label-Free Quantitation for Clinical Proteomics
, 2016 Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be ...
Robert Moulder +2 more
openaire +3 more sources