Results 201 to 210 of about 440,700 (256)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Label-Free Differentiation of Antimicrobial Resistance Groups Using Raman Spectroscopy. [PDF]

Anal Chem
Pistiki A, Ryabchykov O, Wagenhaus A, Bocklitz TW, Deinhardt-Emmer S, Löffler B, Rösch P, Popp J.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry. [PDF]

Anal Chem
Rimmer MA, Wang J, Ranathunge TA, Shi Z, Li Y, Lee D, Chai SC, Lin W, Twarog NR, Ansari AZ, Shelat AA, Young BM, Chen T, Yang L.   +13 more
europepmc   +1 more source

Machine Learning-Driven Nanopore Sensing for Quantitative, Label-Free miRNA Detection. [PDF]

Small Methods
Koch C, Sakthimani S, Noakes VM, Cretu M, Newman D, Gutierrez R, Bruce M, Gorelik J, Guerra N, Edel JB, Ivanov AP.   +10 more
europepmc   +1 more source

Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets. [PDF]

Sensors (Basel)
Coughlan MF, Zhang L, Khan U, Zhang X, Upputuri PK, Glyavina M, Zakharov YN, Qiu L, Perelman LT.   +8 more
europepmc   +1 more source

Rapid trapping and label-free optical characterization of single nanoscale extracellular vesicles and nanoparticles in solution. [PDF]

Light Sci Appl
Hong I, Hong C, Anyika T, Zhu G, Ugwu M, Higginbotham JN, Franklin JL, Coffey R, Ndukaife JC.   +8 more
europepmc   +1 more source