Results 71 to 80 of about 440,393 (255)
Tools for Label-free Peptide Quantification
Molecular & Cellular Proteomics, 2013 The increasing scale and complexity of quantitative proteomics studies complicate subsequent analysis of the acquired data. Untargeted label-free quantification, based either on feature intensities or on spectral counting, is a method that scales particularly well with respect to the number of samples.
S. Nahnsen +3 more
openaire +3 more sources
Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations
FEBS Letters, EarlyView.The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas +6 more
wiley +1 more source
Label-free and label-based electrochemical detection of disease biomarker proteins
ADMET and DMPKIntroduction: Biosensors, analytical devices integrating biological sensing elements with physicochemical transducers, have gained prominence as rapid and convenient tools for monitoring human health status using biochemical analytes.
Tias Febriana Hanifa Lestari +7 more
doaj +1 more source
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes
FEBS Letters, EarlyView.Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez +2 more
wiley +1 more source
Label-Free Quantitation for Clinical Proteomics
, 2016 Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be ...
Robert Moulder +2 more
openaire +3 more sources
NOVEL FREE LABEL BOTULINUM APTASENSOR BASED ON CAPACITANCE METHOD
Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2016 A feasible and fast method to fabricate Botulinum neurotoxin serotype A (BoNT/A) biosensor was investigated by graphene-carbon paste nanocomposite electrode. In the present work, the graphene was synthesis by chemical method and characterized by SEM. The
JAMAL RASHIDIANI +3 more
doaj
Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor
Bioengineering, 2015 A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions.
Varun Lingaiah Kopparthy +2 more
doaj +1 more source
Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques
Advanced Science, 2020 The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface‐enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be
Xia Zhou +8 more
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source