Results 91 to 100 of about 403,277 (275)

Multimodal nonlinear microscopy: A powerful label-free method for supporting standard diagnostics on biological tissues [PDF]

Journal of Innovative Optical Health Sciences, 2014
The large use of nonlinear laser scanning microscopy in the past decade paved the way for potential clinical application of this imaging technique.
Riccardo Cicchi, Francesco Saverio Pavone   +1 more
doaj   +1 more source

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel [PDF]

, 2013
Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in ...
Attila-Gergely Vegh, Csilla Gergely, Elodie Middendorp, Frederic Cuisinier, Hamideh Salehi, Ivan Panayotov, Pierre-Yves Collart Dutilleul, Sathish Ramakrishnan   +7 more
core   +2 more sources

Label-free nonlinear optical imaging of mouse retina

Biomedical Optics Express, 2015
A nonlinear optical (NLO) microscopy system integrating stimulated Raman scattering (SRS), two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) was developed to image fresh mouse retinas. The morphological and functional details of various retinal layers were revealed by the endogenous NLO signals.
He, Sicong, Ye, Cong, Sun, Qiqi, Leung, Christopher K.S., Qu, Jianan   +4 more
openaire   +3 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Intravital imaging by simultaneous label-free autofluorescence-multiharmonic microscopy

Nature Communications, 2018
Label-free and real-time visualization of the tumor microenvironment is attractive but challenging. Here the authors present an approach for simultaneous autofluorescence functional imaging and second/third harmonic generation imaging of structural ...
Sixian You, Haohua Tu, Eric J. Chaney, Yi Sun, Youbo Zhao, Andrew J. Bower, Yuan-Zhi Liu, Marina Marjanovic, Saurabh Sinha, Yang Pu, Stephen A. Boppart   +10 more
doaj   +1 more source

Coherent Raman spectro-imaging with laser frequency combs

, 2013
Optical spectroscopy and imaging of microscopic samples have opened up a wide range of applications throughout the physical, chemical, and biological sciences.
Bernhardt, Birgitta, Guelachvili, Guy, Holzner, Simon, Hänsch, Theodor W., Ideguchi, Takuro, Picqué, Nathalie   +5 more
core   +1 more source

Handheld optical-resolution photoacoustic microscopy [PDF]

, 2017
Optical-resolution photoacoustic microscopy (OR-PAM) offers label-free in vivo imaging with high spatial resolution by acoustically detecting optical absorption contrasts via the photoacoustic effect. We developed a compact handheld OR-PAM probe for fast
Li, Lei, Lin, Li, Shi, Junhui, Wang, Lidai, Wang, Lihong V., Xu, Song, Yao, Junjie, Zhang, Pengfei, Zou, Jun   +8 more
core   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Review on Photoacoustic Monitoring after Drug Delivery: From Label-Free Biomarkers to Pharmacokinetics Agents

Pharmaceutics
Photoacoustic imaging (PAI) is an emerging noninvasive and label-free method for capturing the vasculature, hemodynamics, and physiological responses following drug delivery.
Jiwoong Kim, Seongwook Choi, Chulhong Kim, Jeesu Kim, Byullee Park   +4 more
doaj   +1 more source

Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments

Nature Communications, 2020
Quantitative phase imaging suffers from a lack of specificity in label-free imaging. Here, the authors introduce Phase Imaging with Computational Specificity (PICS), a method that combines phase imaging with machine learning techniques to provide ...
Mikhail E. Kandel, Yuchen R. He, Young Jae Lee, Taylor Hsuan-Yu Chen, Kathryn Michele Sullivan, Onur Aydin, M. Taher A. Saif, Hyunjoon Kong, Nahil Sobh, Gabriel Popescu   +9 more
doaj   +1 more source