Results 221 to 230 of about 403,277 (275)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Discontinuity third harmonic generation microscopy for label-free imaging and quantification of intraepidermal nerve fibers. [PDF]

Cell Rep Methods
Wu PJ, Tseng HC, Chao CC, Liao YH, Yen CT, Lin WY, Hsieh ST, Sun WZ, Sun CK.   +8 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Author Correction: Plasmonic nano-aperture label-free imaging of single small extracellular vesicles for cancer detection. [PDF]

Commun Med (Lond)
Ohannesian N, Mallick MS, He J, Qiao Y, Li N, Shaitelman SF, Tang C, Shinn EH, Hofstetter WL, Goltsov A, Hassan MM, Hunt KK, Lin SH, Shih WC.   +13 more
europepmc   +1 more source

Navigating new norms: a systematic review of factors for the development of effective digital tools in higher education

FEBS Open Bio, EarlyView.
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman, Jessica Haines, Connie Pritchard, Stephen Rutherford, Nigel Francis   +4 more
wiley   +1 more source

Fast Fourier ptychographic quantitative phase microscopy for in vitro label-free imaging. [PDF]

Biomed Opt Express
Wu R, Luo Z, Liu M, Zhang H, Zhen J, Yan L, Luo J, Wu Y.   +7 more
europepmc   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

Label-Free Imaging of Inflammation at the Level of Single Cells in the Living Human Eye. [PDF]

Ophthalmol Sci
Rui Y, Zhang M, Lee DMW, Snyder VC, Raghuraman R, Gofas-Salas E, Mecê P, Yadav S, Tiruveedhula P, Grieve K, Sahel JA, Errera MH, Rossi EA.   +12 more
europepmc   +1 more source

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

3DMOUSEneST: a volumetric label-free imaging method evaluating embryo-uterine interaction and decidualization efficacy.

Development
Savolainen A, Kapiainen E, Ronkainen VP, Izzi V, Matzuk MM, Monsivais D, Prunskaite-Hyyryläinen R.   +6 more
europepmc   +1 more source