Results 111 to 120 of about 883,522 (286)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Cost-Per-Responder Analysis for Tralokinumab and Dupilumab in Combination with Topical Corticosteroids in Patients with Moderate-To-Severe Atopic Dermatitis

Dermatology and Therapy
Introduction Atopic dermatitis (AD) is a chronic inflammatory skin disease, and patients with moderate-to-severe AD may require treatment with biologics in combination with topical corticosteroids (TCS).
Marni Wiseman, Marco Benvenuto, Liv Strømkjær, Asger Paludan-Müller, Lasse Ryttig, Anne Sohrt Petersen, Andreas Wollenberg   +6 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Design and Optimization of Metropolitan Area Networks using Multi-Protocol Label Switching

Majlesi Journal of Electrical Engineering
Looking in to the modern technology, we see new inventions and innovations which are unique. As an example, over the past years, new technology has been created in computer networks of which metropolitan area network (MAN) has had a noticeable advance in
Alireza Emamjomeh, Hossein Emami, Ali Hashemi   +2 more
doaj  

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Flow-splitting algorithm of MPLS single label based on programmable router

Tongxin xuebao, 2014
To increase the QoS (quality of service) of data transmission, a novel flow-splitting algorithm, multipathMPLS, was proposed, which combined three technologies of MPLS (multi protocol label switching), concurrent multi-path and programmable router ...
Lai-quan HAN, Jin-kuan WANG, Xing-wei WANG   +2 more
doaj   +2 more sources

Label switching using the IPv6 address hierarchy

, 2000
Current label switching protocols can use routing, address, and address hierarchy information to group flows for cut-throughs that bypass IP forwarding. This paper examines a label switching solution that uses the IP version 6 (IPv6) address structure to
P Boustead (20184891), Jose Chicharo (19582207)   +1 more
core   +1 more source

Multidimensional data analysis and classification using SMIAL

Communications Biology
Label-free imaging enables multidimensional data acquisition across spectral and temporal domains and is increasingly used in life sciences. However, users working with multidimensional label-free data often lack the programming expertise needed to build
Aline Knab, Shannon Handley, Xiaohu Xu, Max Mackevicius, Ayad G. Anwer, Jared M. Campbell, Yuan Tian, Ewa M. Goldys, Akanksha Bhargava   +8 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source