Results 41 to 50 of about 55,056 (314)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

open access: yesMolecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen   +11 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Gender and class : women's working lives in a dormitory labor regime in China

open access: yes, 2012
The thirty years since Women on the Line has witnessed great achievement in the literature of gender and work both in the West and Global South. There was a booming literature since the 1970s and 1980s in the fields of sociology, anthropology, women ...
Pun, N
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

open access: yesFEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Inside the early twentieth century working class family : kin, work and neighborhood

open access: yes, 2021
Shannon Kirkwood, historian and independent scholar, delivers a talk called "Inside the early twentieth century working class family: kin, work and neighborhood", containing material from her upcoming book.
Kirkwood, Shannon R.
core  

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

open access: yesFEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell   +8 more
wiley   +1 more source

David Montgomery In Brazil

open access: yes, 2015
David Montgomery's semester as a Fulbright lecturer at the State University of Campinas, Brazil, in 1986 proved quite memorable. Bela Bianca, a member of the anthropology department who had known David and Marty when she was on a postdoctoral grant at ...
Batalha C., Hall M.M.
core   +1 more source

Eradicating child poverty in Britain: welfare reform and children since 1997 [PDF]

open access: yes, 2001
Over the past 20 years the incidence of relative poverty among Britain’s children has tripled. These changes are related to increased earnings inequality, growth in the number of single (lone) parent households, and an increased share of households with ...
Gregg, P.   +5 more
core   +1 more source

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