Results 191 to 200 of about 2,504,342 (301)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

AAV-based vectors for human diseases modeling in laboratory animals. [PDF]

Front Med (Lausanne)
Aliev TI, Yudkin DV.
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Prospects of Electrocorticography in Neuropharmacological Studies in Small Laboratory Animals. [PDF]

Brain Sci
Sysoev YI, Okovityi SV.
europepmc   +1 more source

The outcome of early life status epilepticus-lessons from laboratory animals. [PDF]

Epilepsia Open, 2023
Kubová H, Mikulecká A, Mareš P.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The Veterinary Consortium for Research Animal Care and Welfare Survey on Revisions to the Eighth Edition of the Guide for the Care and Use of Laboratory Animals. [PDF]

J Am Assoc Lab Anim Sci
Storves KP, Talcott MR, Wallace JM, Bennett BT, Makaron LM, Clemons D, Hugh Chip Price V, Cohen JK, Hasenau JJ, Freed CK, Leland SE.   +10 more
europepmc   +1 more source

Age-Dependent Changes in the Effects of Androgens on Female Metabolic and Body Weight Regulation Systems in Humans and Laboratory Animals. [PDF]

Int J Mol Sci, 2023
Iwasa T, Noguchi H, Tanano R, Yamanaka E, Takeda A, Tamura K, Aoki H, Sugimoto T, Sasada H, Maeda T, Minato S, Yamamoto S, Inui H, Kagawa T, Yoshida A, Mineda A, Nii M, Kinouchi R, Yoshida K, Yamamoto Y, Kaji T.   +20 more
europepmc   +1 more source

High Parasitic Infections in the Laboratory Animals: Complications for the Research Outcomes and Zoonotic Importance

Iranian Journal of Parasitology
Background: Laboratory animals are widely used for medical research; hence, their health status is a determinant of the outcome and success of the research.
Seyed Reza Mirbadie, Salman Zafari, Naghmeh Dastan, Mohammad Fallah   +3 more
doaj  

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source